Article
  • Formation of Fibroin Microspheres by Molecular Cohesion and Association in Water-in-oil Emulsion
  • Lee JS, Kim JY, Hur W
  • W/O 에멀젼에서 피브로인 분자의 회합 및 응집에 의한 미세구의 형성
  • 이진실, 김지영, 허원
Abstract
Fibroin is an insoluble protein polymer of silk fiber but undergo solubilization in a hot salt solution. The fibroin-salt solution was dialyzed to obtain an unstable aqueous fibroin, eventually turned to gel. Molecular association of aqueous fibroin and their growth were detected by dynamic light scattering. Fibroin microspheres were prepared by dehydration of W/O emulsion from aqueous fibroin, mixed with decane and detergents. Scanning electron microscopy showed that fibroin microspheres have the smooth surface. The fibroin microspheres remain insoluble in water as well as in saline without any significant swelling. FTIR and XRD were used to detect the presence of β-sheet structure formed by hydrogen bonds between fibroin molecules in an orderly manner.

실크섬유의 구성단백질인 피브로인은 물에 녹지 않으나 고온의 염용액에 녹는다. 염용액을 투석하면 피브로인 수용액이 얻어지고, 시간이 경과하면 젤이 형성된다. 본 연구에서는 젤 형성 중간단계에 존재하는 피브로인 분자회합체를 광산란법으로 분석하였고, 시간의 경과에 따라 그 크기가 증가하는 것을 확인하였다. 피브로인 수용액에 데칸과 계면활성제를 섞어 유상수적(W/O)형의 에멀젼을 만들고 감압 건조시켜 미세구를 제조하였다. 피브로인 미세구는 매끄러운 표면의 구형을 유지하고 있으며, 수용액이나 식염수에 보관해도 불용성 상태를 유지하였다. 미세구의 불용성과 형태 안정성에 기여하는 피브로인 분자사슬의 β-sheet 구조와 결정성 영역을 적외선 분광 스펙트럼과 X선 회절 분석을 이용하여 확인하였다.

Keywords: fibroin; microsphere; β-sheet structure

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  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2016; 40(6): 865-870

    Published online Nov 25, 2016

  • 10.7317/pk.2016.40.6.865
  • Received on Apr 11, 2016
  • Accepted on Jun 27, 2016