Article
  • Evaluation of Osteogenesis on Duck's Feet Derived Collagen and Demineralized Bone Particles Sponges
  • Cha JG, Cha SR, Lee DH, Shin JH, Song JE, Suh DS, Park CH, Khang G
  • 오리발 유래 콜라겐과 탈미네랄화된 골분 스펀지에서 골 분화능력평가
  • 차재근, 차세롬, 이대훈, 신재훈, 송정은, 서동삼, 박찬흠, 강길선
Abstract
In this study, duck's feet derived collagen (DC) and demineralized bone particles (DBP) sponges were prepared by mixing both the components on the ratio of 3:1, 1:1, and 1:3. The physio-chemical properties of the sponges were examined by measuring compressive strength, porosity, SEM, etc. In vitro biological characterizations such as cell attachment, proliferation and osteogenic differentiation of the as-prepared sponges were studied by inoculating the bone marrow-derived stem cells (BMSC) in ratio-specific DC/DBP sponges followed by SEM, MTT, ALP, RT-PCR & histological analysis by staining. We found that the 1:1 DC/DBP sponges displayed superior osteogenesis, thus can be envisioned as a potential, biomaterial for regeneration of bone.

본 연구에서는 오리발 콜라겐(duck's feet derived collagen, DC)과 탈미네랄화된 골분(demineralized bone particles, DBP)을 3:1, 1:1, 1:3 비율로 혼합하여 스펀지를 제조하였다. 제조한 스펀지의 특성분석을 위해 압축강도, 다공성, SEM 등을 측정하였으며, 골수 유래 줄기세포(BMSC)를 비율별 DC/DBP 스펀지에 파종하여, 세포 부착, 증식 및 골 분화를 확인하고자, SEM, MTT, ALP, RT-PCR 및 조직학적 염색을 통한 분석을 실시하였다. 그 결과, 1:1 DC/DBP 스펀지에서 골 분화 효과가 우수한 것을 확인하였으며, 이를 골 재생을 위한 생체재료로 활용할 수 있을 것으로 기대된다.

Keywords: duck's feet derived collagen (DC); demineralized bone particle (DBP); bone marrow stem cells (BMSc) osteogenesis

References
  • 1. Yang S, Leong KF, Du Z, Chua CK, Tissue Eng., 7, 679 (2001)
  •  
  • 2. Humacher DW, Biomaterials, 21, 2529 (2000)
  •  
  • 3. Lee KY, Mooney DJ, Am. Chem. Soc., 101, 1069 (2001)
  •  
  • 4. Fleming JE, Cornell CN, Muschler GF, Ortop. Clin. N. Am., 31, 357 (2000)
  •  
  • 5. Partridge K, Yang XB, Clarke NMP, Okubo Y, Bessho K, Sebald W, Howdle SM, Shakesheff KM, Oreffo ROC, Biochem. Biophys. Res. Commun., 292(1), 144 (2002)
  •  
  • 6. Yang SH, Hsu CK, Wang KC, Hou SM, Lin FH, J. Biomed. Mater. Res. B, Appl. Biomater., 74, 468 (2005)
  •  
  • 7. Lu HH, Kofron MD, El-Amin SF, Attawia MA, Laurencin CT, Biochem. Biophys. Res. Commun., 305(4), 882 (2003)
  •  
  • 8. Joyce ME, Roberts AB, Sporn MB, Bolander M, J. Cell. Biol., 110, 2195 (1990)
  •  
  • 9. Steinbrech DS, Mehrara BJ, Rowe NM, Dudziak ME, Saadeh PB, Gittes GK, Longaker MT, Ann. Plast. Surg., 42, 481 (1999)
  •  
  • 10. Canalis E, McCarthy TL, Centrella M, J. Cell Physiol., 140, 530 (1989)
  •  
  • 11. Ye Q, Ohsaki K, Li K, Li DJ, Zhu CS, Ogawa T, Tenshin S, Takano-Yamanoto T, Auris Nasus Larynx, 28, 131 (2001)
  •  
  • 12. Liu C, Xia Z, Czernuszka JT, Chem. Eng. Res. Des., 85(A7), 1051 (2007)
  •  
  • 13. Singer AJ, Clark RA, N. Engl. J. Med., 341, 738 (1999)
  •  
  • 14. Caliari SR, Ramirez MA, Harely BAC, Biomaterials, 32, 8990 (2011)
  •  
  • 15. Capito RM, Spector M, Gene Therapy, 14, 721 (2007)
  •  
  • 16. Murray JC, Stingl G, Kleinman HK, Martin GR, Katz SI, J. Cell Biol., 80, 197 (1979)
  •  
  • 17. Chong EJ, Phan TT, Lim IJ, Zhang YZ, Bay BH, Ramakrishna S, Lim CT, Acta Biomater., 3, 321 (2007)
  •  
  • 18. Oliver RF, Barker H, Cooke A, Grant RA, Biomaterials, 3, 38 (1982)
  •  
  • 19. Lynch SR, Colvin RB, Antoniades HN, J. Clin. Invest., 84, 640 (1989)
  •  
  • 20. Kropp BP, Eppley BL, Prevel CD, Rippy MK, Harruff RC, Badylak SF, Adams MC, Rink RC, Keating MA, Urology, 46, 396 (1995)
  •  
  • 21. Mizuno S, Glowacki J, Biomaterials, 17, 1819 (1996)
  •  
  • 22. Cha SR, Jeong HK, Kim SY, Kim EY, Song JE, Park CH, Kwon SY, Khang G, Polym. Korea, 39(3), 493 (2015)
  •  
  • 23. Lee WK, Ichi T, Ooya T, Yamamoto T, Katoh M, Yui N, J. Biomed. Mater. Res. Part A, 67A, 1087 (2003)
  •  
  • 24. Ko HA, Kim HM, Ha HJ, Kim R, Jang JE, Kim KH, Lee JH, Song JS, Yoon H, Lee OJ, Lee D, Park CH, Khang G, Inter. J. Tissue Regen., 3, 103 (2012)
  •  
  • 25. Farrugia BL, Whitelock JM, Jung M, McGrath B, O’Grady RL, McCarthy SJ, Lord MS, Biomaterials, 35, 1462 (2014)
  •  
  • 26. Asheley RA, Palmer BW, Schultz AD, Woodson BW, Roth CC, Routh JC, Fung KM, Frimberger D, Lin HK, Kropp BP, Tissue Eng. Part A, 15, 3241 (2009)
  •  
  • 27. Bucktrout SLB, Liordella MM, Zhou X, Anthony B, Rosenthal W, Luche H, Fehling HJ, Bluestone JA, Immunity, 39, 949 (2013)
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2016; 40(6): 858-864

    Published online Nov 25, 2016

  • 10.7317/pk.2016.40.6.858
  • Received on Apr 4, 2016
  • Accepted on Jul 26, 2016