Article
  • Comparison between Poly(lactic-co-glycolic acid) Films Contained Natural Polymers on Adhesion and Proliferation of Schwann Cells
  • Ko HA, Jang JE, Kim H, Park CH, Kwon SY, Chung JW, Khang G
  • 천연 고분자가 함유된 락타이드 글리콜라이드 공중합체 필름에서 슈반세포의 부착과 증식 거동 비교평가
  • 고현아, 장지은, 김형석, 박찬흠, 권순용, 정진화, 강길선
Abstract
This study was designed to find an appropriate biomaterial to proliferate Schwann cell (SC). Poly(lactic-coglycolic acid) (PLGA) films mixed with demineralized bone particle (DBP), small intestine submucosa (SIS), and silk were fabricated by a solvent casting method. Analysis of MTT, SEM and RT-PCR were performed to confirm adhesion and proliferation of SC. Contact angle of films was assayed for hydrophilicity of films. We confirmed that PLGA/DBP 20% film showed higher hydrophilicity, promoted adhesion and proliferation of SC than other films. It was concluded that PLGA/DBP film can be applied for the scaffold biomaterials for the regeneration of central nerve system.

본 연구에서는 신경세포인 슈반세포(SC)의 증식에 가장 적합한 생체재료를 연구하였다. 락타이드 글리콜라이드 공중합체(PLGA)에 탈미네랄 골분(demineralized bone particle, DBP), 소장점막하조직(small intestine submucosa, SIS), 그리고 실크를 각각 20% 첨가하여, 용매 증발법으로 각각의 필름을 제조하고, SC세포의 부착과 증식을 확인하기 위해 MTT, SEM 그리고 RT-PCR 분석을 실시하였다. 또한 필름의 친수성을 확인하기 위해 접촉각을 측정하였다. 분석 결과, PLGA/DBP 20% 필름에서 높은 친수성을 보였으며, SC의 부착과 증식률이 다른 군에 비해 크게 증가한 것을 확인할 수 있었다. 따라서 PLGA/DBP 필름은 중추신경재생 재료로 활용할 수 있을 것으로 사료된다.

Keywords: schwann cell; demineralized bone particle (DBP); small intestine submucosa (SIS); silk; PLGA hybridized film.

References
  • 1. Xu XM, Chen A, Guenard V, Kleitman N, Bunge MB, Neurocytol, 26, 1 (1997)
  •  
  • 2. Salzer JL, Bunge RP, J. Cell Biology, 84, 739 (1980)
  •  
  • 3. Atala A, J. Endourol., 14, 49 (2000)
  •  
  • 4. Zhang N, Yan H, Wen X, Brain Res., 49, 48 (2005)
  •  
  • 5. Khang G, Jeon JH, Cho JC, Rhee JM, Lee HB, Polym.(Korea), 23(6), 861 (1999)
  •  
  • 6. Holland SJ, Tighe BJ, Could PL, J. Control. Res., 4, 155 (1986)
  •  
  • 7. Iwasaki Y, Sawada S, Nakabayashi N, Khang G, Lee HB, Ishihara K, Biomaterials, 20, 2185 (1999)
  •  
  • 8. Khang G, Lee SJ, Lee JH, Kim YS, Lee HB, Macromol. Res., 8, 276 (2000)
  •  
  • 9. Huggins CB, Urist MR, Science, 150, 893 (1970)
  •  
  • 10. Urist MR, Science, 167, 896 (1970)
  •  
  • 11. Hogan BL, Harvey Lect., 92, 83 (1996)
  •  
  • 12. Ebenbal T, Bengtsson H, Soderstrom S, J. Neurosci. Res., 51, 139 (1998)
  •  
  • 13. Lonn P, Zaia K, Israelsson C, Althini S, Usoskin D, Kylberg A, Ebendal T, Neurochem. Res., 30, 753 (2005)
  •  
  • 14. Khang G, Rhee JM, Shin P, Kim IY, Lee B, Lee SJ, Lee YM, Lee HB, Lee I, Macromol. Res., 10(3), 158 (2002)
  •  
  • 15. Badylak SF, Record R, Lindberg K, J. Biomater. Sci. Polym. Ed., 9, 863 (1998)
  •  
  • 16. Park KS, Jin CM, Yun SJ, Hong KD, Kim SH, Kim MS, Rhee JM, Khang G, Lee HB, Polym.(Korea), 29(5), 501 (2005)
  •  
  • 17. Chen J, Altman GH, Karageorgiou V, Horan R, Collette A, Volloch V, J. Biomed. Mater. Res., 67, 559 (2003)
  •  
  • 18. Jin HJ, Kaplan DL, Nature, 424, 1057 (2003)
  •  
  • 19. Jin HJ, Fridrikh SV, Rutledge GC, Kaplan DL, Biomacromolecules, 3(6), 1233 (2002)
  •  
  • 20. Soong HK, Kenyon KR, Ophthalmology, 91, 479 (1984)
  •  
  • 21. Panilaitis B, Altman GH, Chen J, Jang HJ, Karageorgiou V, Kaplan DL, Biomaterials, 24, 3079 (2003)
  •  
  • 22. Meinel L, Betz O, Fajardo R, Hofmann S, Nazarian A, Bone, 39, 922 (2006)
  •  
  • 23. Kim HL, Yoo H, Park HJ, Kim YG, Lee D, Kang YS, Khang G, Polym.(Korea), 35(1), 7 (2011)
  •  
  • 24. Porter S, Clark MB, Glaser L, Bunge RP, J. Neurosci., 6, 3070 (1986)
  •  
  • 25. Morrissey TK, Kleitman N, Bunge RP, J. Neurosci., 11, 2433 (1991)
  •  
  • 26. Kim HL, Kim SJ, Yoo H, Hong M, Lee D, Khang G, Int. J. Tissue Regen., 1, 81 (2010)
  •  
  • 27. Gu BK, Kim MS, Park SJ, Kim C, Int. J. Tissue Regen., 2, 83 (2011)
  •  
  • 28. Yamamoto M, Tabata Y, Int. J. Tissue Regen., 4, 36 (2013)
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2014; 38(2): 164-170

    Published online Mar 25, 2014

  • Received on Sep 17, 2013
  • Accepted on Nov 6, 2013