Article
  • Mechanical Property and Cell Compatibility of Silk/PLGA Hybrid Scaffold; In Vitro Study
  • Song Y, Yoo H, Eum S, Kim OY, Yoo SC, Kim HE, Lee D, Khang G
  • 실크/PLGA 하이브리드 지지체의 기계적 물성과 세포친화력; in vitro 연구
  • 송이슬, 유한나, 엄신, 김온유, 유석철, 김형은, 이동원, 강길선
Abstract
The design of new bioactive scaffolds offering physiologic environment for tissue formation is an important frontier in biomaterials research. In this study, we performed compressive strength, water-uptake ability, and SEM analysis for physical property assessment of 3-D silk/PLGA scaffold, and investigated the adhesion, proliferation, phenotype maintenance, and inflammatory responses of RAW 264.7 and NIH/3T3 for cell compatibility. Scaffolds were prepared by the solvent casting/salt leaching method and their compressive strength and water-uptake ability were excellent at 20 wt% silk content. Result of cell compatibility assay showed that inflammatory responses distinctly decreased, and initial adhesion and proliferation were maximized at 20 wt% silk content. In conclusion, we suggest that silk/PLGA scaffolds may be useful to tissue engineering applications.

조직 형성을 위한 생리학적 환경을 제공하는 새로운 생활성 지지체의 디자인은 생체재료 연구에서 중요한 분야이다. 본 연구에서는 3차원적 실크/PLGA 지지체의 물성평가를 위해 압축강도, 수분친화도, SEM 분석을 하였으며, 세포친화성 평가를 위해 RAW 264.7과 NIH/3T3의 부착, 증식 및 표현형유지와 염증반응에 미치는 영향을 조사하였다. 지지체는 용매 캐스팅/염 추출법으로 제조하였고, 압축강도, 수분친화도 면에서 실크 함량이 20wt%에서 우수함을 확인했으며, 표면의 거침도를 높여 세포부착에 긍정적인 구조임을 확인하였다. 세포친화성 분석 결과, 실크함량이 20 wt%인 실크/PLGA 지지체에서 높은 초기부착도 및 증식률을 보였으며, 실크함량이 20wt%에서 염증반응이 눈에 띄게 감소함을 확인하였다. 조직공학적 응용에 실크/PLGA 지지체가 유용할 것이라 판단하였다.

Keywords: PLGA; silk; hybrid scaffold; cell compatibility.

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  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
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This Article

  • 2011; 35(3): 189-195

    Published online May 25, 2011

  • Received on Jul 20, 2010
  • Accepted on Nov 16, 2010