Article
  • Preparation and Characterization of Electrospun Nanofibers Containing Natural Antimicrobials
  • Kim YJ, Kim SN, Kwon OK, Park MR, Kang IK, Lee SG
  • 천연 항균물질 함유 나노섬유의 제조 및 특성분석
  • 김영진, 김상남, 권오경, 박미란, 강인규, 이세근
Abstract
The fabrication of PHBV nanofibers containing various plant polyphenols by electrospinning has been examined. It has been found that the average diameters of fibers increased by the adding of polyphenols. The resulting fibers exhibited a uniform diameter ranging from 340 to 450 nm. As the concentration of polyphenols increased, the diameter of fibers increased due to the hydrogen bonding interaction between the ester groups of PHBV and hydroxyl groups of polyphenols. The interaction between PHBV and polyphenols, which forms a complex together in solution, was verifed by UV measurement. ATR-FTIR analysis confirmed the existence of the hydrogen bonding interaction. The semicrystalline structure of the PHBV nanofiber was observed from XRD pattern. The crystallinity of PHBV nanofibers was increased by the adding of polyphenols. PHBV nanofibers containing polyphenols showed superior antimicrobial activities.

전기방사법으로 식물성 폴리페놀을 함유하는 PHBV 나노섬유를 제조하였으며, 얻어진 나노섬유의 평균직경은 340-450 nm였다. 폴리페놀의 첨가에 의해 나노섬유의 직경이 증가하였으며 폴리페놀의 첨가량도 나노섬유의 직경 변화에 영향을 미쳤다. 이는 PHBV와 폴리페놀 사이의 수소결합에 기인하는 것으로 확인되었다. 제조된 나노섬유를 이용한 ATR-FTIR 분석 결과 PHBV와 폴리페놀 사이에 수소결합이 존재하는 것을 확인할 수 있었고, XRD 분석 결과 폴리페놀의 첨가에 의해 PHBV 나노섬유의 결정성이 높아졌다. 이들 폴리페놀을 함유하는 나노섬유는 우수한 항균특성을 보였다.

Keywords: electrospinning; PHBV; nanofiber; plant polyphenol; natural antimicrobial.

References
  • 1. Hammerstone JF, Lazarus SA, Schmitz HH, J. Nutr., 130, 2086 (2000)
  •  
  • 2. Lopez M, Martinez F, Del Valle C, Orte C, Miro M, J. Chromatogr., 922, 359 (2001)
  •  
  • 3. Shahidi F, Naczk MPhenolic in Food and Nutraceuticals: Sources, Applications, and Health Effects, CRC Press, Boca Raton, FL (2003)
  •  
  • 4. Jankun J, Selman SH, Swiercz R, Skrzypczakjankun E, Nature, 387(6633), 561 (1997)
  •  
  • 5. Di Carlo G, Mascolo N, Izzo AA, Capasso F, Life Sci., 65, 337 (1999)
  •  
  • 6. Samuelsen AB, J. Ethnopharmacol., 71, 1 (2000)
  •  
  • 7. Ren W, Qiao Z, Wang H, Zhang L, Med. Res. Rev., 23, 519 (2003)
  •  
  • 8. Huang W, Liu MZ, Li Y, Tan Y, Yang GF, Bioorgan. Med. Chem., 15, 5191 (2007)
  •  
  • 9. Cowan MM, Clin. Microbiol. Rev., 12, 564 (1999)
  •  
  • 10. Hemaiswarya S, Kruthiventi AK, Doble M, Phytomedicine, 15, 639 (2008)
  •  
  • 11. Guan HY, Shao CL, Wen SB, Chen B, Gong J, Yang XH, Mater. Chem. Phys., 82(3), 1002 (2003)
  •  
  • 12. Yoshimoto H, Shin YM, Terai H, Vacanti JP, Biomaterials, 24, 2077 (2003)
  •  
  • 13. Zeng J, Xu X, Chen X, Liang Q, Bian X, Yang L, Jing X, J. Control. Release, 92, 227 (2003)
  •  
  • 14. Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S, Compos. Sci. Technol., 63, 2223 (2003)
  •  
  • 15. Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH, Biomaterials, 25, 1289 (2004)
  •  
  • 16. Huang L, McMillan RA, Apkarian RP, Pourdeyhimi B, Conticello VP, Chaikof EL, Macromolecules, 33(8), 2989 (2000)
  •  
  • 17. Caruso RA, Schattka JH, Greiner A, Adv. Mater., 13(20), 1577 (2001)
  •  
  • 18. Chen ZH, Foster MD, Zhou WS, Fong H, Reneker DH, Resendes R, Manners I, Macromolecules, 34(18), 6156 (2001)
  •  
  • 19. Li WJ, Laurencin T, Caterson EJ, Tuan RS, Ko FK, J. Biomed. Mater. Res., 60, 613 (2002)
  •  
  • 20. Cho SH, Oh SH, Lee JH, J. Biomater. Sci. Polym. Ed., 13, 933 (2005)
  •  
  • 21. Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)
  •  
  • 22. Langer R, Vacanti JP, Science, 260, 920 (1993)
  •  
  • 23. Ginaalska G, Kowalczuk D, Osinska M, Intern. J. Pharm., 288, 131 (2008)
  •  
  • 24. Pouton CW, Akhtar S, Adv. Drug Del. Rev., 18, 133 (1996)
  •  
  • 25. Kose GT, Kenar H, Hasırcı H, Hasırcı V, Biomaterials, 24, 1949 (2003)
  •  
  • 26. Ye WJ, Leung MF, Xin J, Kwong TL, Lee DKL, Li P, Polymer, 46(23), 10538 (2005)
  •  
  • 27. Kim YJ, Uyama H, Kobayashi S, Macromolecules, 36(14), 5058 (2003)
  •  
  • 28. Kim YJ, Uyama H, Kobayashi S, Macromol. Biosci., 4, 497 (2004)
  •  
  • 29. Padermshoke A, Katsumoto Y, Sato H, Ekgasit S, Noda I, Ozaki Y, Polymer, 45(19), 6547 (2004)
  •  
  • 30. Yoshie N, Saito M, Inoue Y, Macromolecules, 34(26), 8953 (2001)
  •  
  • 31. Tsuchiya H, Sato M, Miyazaki T, Fujiwara S, Tanigaki S, Ohyama M, Tanaka T, Iinuma M, J. Ethnopharmacol., 50, 27 (1996)
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2009; 33(4): 307-312

    Published online Jul 25, 2009

  • Received on Dec 30, 2008
  • Accepted on Mar 3, 2009