Article
  • Targeted Gene Delivery of the Cationic Lipid Emulsion System Containing Folate-PEG-DPPE
  • Kwon SK, Jeong KH, Kim YJ
  • Folate-PEG-DPPE를 포함하는 양이온성 지질 에멀젼 시스템의 표적화 유전자 전달
  • 권상규, 정관호, 김영진
Abstract
A cationic lipid emulsion containing 1,2-dioleoyl-3-trimethylammonium-propane(DOTAP), Tween80, squalene has been prepared as a gene delivery system. In order to increase the transfection efficiency of gene carrier, folate was used as the tumor-targeting ligand that was attached on PEGDPPE. HeLa and 293 cells were used for the in vitro transfection experiment. HeLa cell is a folatepositive cell line. The mean particle sizes of polymeric lipid system and DNA/lipid complex system were 206.6 nm and 150.5 nm, respectively. The transfection efficiencies of our carriers(4:1(w:w) complex ratio)were 100 times higher than that of DOTAP only emulsion due to the targeting effect of folate.

1,2-Dioleoyl-3-trimethylammonium-propane(DOTAP)와 Tween80, squalene을 포함하는 양이온 성 지질 에멀젼을 기반으로 효율적인 비바이러스성 유전자 전달체를 개발하였다. 유전자 전달체의 발현효율을 증가시키기 위해 암세포에 표적지향성을 가지는 folate를 수식한 PEG-DPPE를 사용하였다. Folate-PEG-DPPE를 포함하는 양이온성 지질 에멀젼으로 HeLa 세포주와 293 세포주에 유전자 형질발현 실험을 하였다. HeLa 세포는 folate에 민감한 세포주이다. 양이온성 지질 에멀젼의 입자크기와 DNA/lipid 복합체의 크기는 각각 206.6 nm와 150.5 nm로 측정되었다. 양이온성 지질 시스템/DNA(4:1(w:w)) 복합체의 유전자 발현효율은 folate의 표적화 영향으로 인해 DOTAP만 있는 에멀젼에 비하여 100배 이상 더 높은 것으로 나타났다.

Keywords: cationic lipid emulsion; folate; PEG; DOTAP; targeted gene delivery.

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  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2009; 33(3): 213-218

    Published online May 25, 2009

  • Received on Nov 27, 2008
  • Accepted on Jan 21, 2009