Article
  • Preparation and Release Behavior of Methoxy poly(ethylene glycol)-poly(L-lactide-co-glycolide) Wafer Containing Albumin
  • Seo KS, Kim MS, Kim K, Cho SH, Lee HB, Khang G
  • 알부민을 함유한 메톡시 폴리(에틸렌 글리콜)-폴리(L-락타이드-co-글리콜라이드) 웨이퍼의 제조 및 방출거동
  • 서광수, 김문석, 김경자, 조선행, 이해방, 강길선
Abstract
A series of methoxy poly(ethylene glycol) (MPEG)-poly(L-lactide-co-glycolide) (PLGA) diblock copolymers were synthesized by ring-opening polymerization of L-lactide and glycolide with carbitol (134 g/mole) or different molecular weights of MPEG (550, 2000, and 5000 g/mole) as an initiator in presence of Sn(Oct)2. The properties of diblock copolymers were characterized by using 1H-NMR, GPC, and XRD. After uniform mixing of block copolymers and 1% albumin bovine-fluorescein isothiocyanate (FITC-BSA) with a freeze miller, the wafers loaded FITC-BSA were fabricated by using a mold with a dimensions of 3 mm × 1mm diameter. The release profiles of FITC-BSA and the pH changes of wafer were examined using pH 7.4 PBS for 30 days at 37 ℃. The release profiles of albumin showed fast initial burst as the molecular weights of MPEG increased. As a result of this study, the release behavior of BSA was controlled with introducing MPEG in the block copolymers.

단백질 전달체로 이용하기 위한 폴리(에틸렌 글리콜)-폴리(락타이드-co-글리콜라이드) 공중합체는 카비톨(134 g/mole)과 550, 2000및 5000 g/mole의 각기 다른 분자량을 가진 메톡시 폴리(에틸렌 글리콜)을 개시제로 하여 촉매인 Sn(Oct)2를 사용하여 L-락타이드와 글리콜라이드의 개환중합에 의해 합성되었다. 1H-NMR, GPC, 그리고 XRD를 이용하여 합성된 블록 공중합체의 특성을 결정하였다. 이식형 웨이퍼를 준비하기 위하여 동결분쇄기를 이용하여 형광이 결합된 1%소 혈청 알부민과 고분자를 균일 혼합 후에 3 mm × 1 mm의 크기로 웨이퍼를 제조하였다. 형광 알부민의 방출 거동과 pH변화는 pH 7.4의 인산염완충용액을 이용하여 37 ℃, 100 rpm의 항온조에서 30일 동안 관찰하였다. 알부민의 방출양은 형광분광기를 통하여 FITC의 강도에 의해 결정되었다. 알부민의 방출 거동은 블록 공중합체내에서 MPEG의 분자량이 증가할수록 빠른 초기 방출을 보였고, PLGA의 분자량을 조절함으로서 약물의 방출기간을 결정할 수 있었다. 이러한 결과로 생분해성 PLGA에 친수성인 MPEG의 도입을 통해 약물의 방출거동을 조절할 수 있었다.

Keywords: block copolymer; MPEG-PLGA; albumin; protein carrier; wafer

References
  • 1. Langer R, Science, 249, 1527 (1990)
  •  
  • 2. Langer R, Ann. Biomed. Eng., 23, 101 (1995)
  •  
  • 3. Cima LG, Vacanti JP, J. Biomech. Eng., 113, 143 (1991)
  •  
  • 4. Langer R, Vacanti JP, Science, 260, 920 (1993)
  •  
  • 5. Cho JC, Khang G, Rhee JM, Kim YS, Lee JS, Lee HB, Korea Polym. J., 7(2), 79 (1999)
  •  
  • 6. Seo KS, Chon SK, Kim MS, Cho SH, Lee HB, Khang G, Polym.(Korea), 28(3), 232 (2004)
  •  
  • 7. Lee HB, Khang G, Cho JC, Rhee JM, Lee JS, Polym. Prepr., 40, 288 (1999)
  •  
  • 8. Khang G, Lee JH, Lee JW, Cho JC, Lee HB, Korea Polym. J., 8(2), 80 (2000)
  •  
  • 9. Lee JS, Shin JH, Jeong JK, Rhee JM, Lee HB, Khang G, Polym.(Korea), 27(1), 9 (2003)
  •  
  • 10. Khang G, Choi HS, Rhee JM, Yoon SC, Cho JC, Lee HB, Korea Polym. J., 8(6), 253 (2000)
  •  
  • 11. Choi JS, Lee SJ, Jang JW, Khang G, Lee YM, Lee B, Lee HB, Polym.(Korea), 27(5), 397 (2003)
  •  
  • 12. Choi HS, Kim SW, Yun DI, Khang G, Rhee JM, Kim YS, Lee HB, Polym.(Korea), 25(3), 334 (2001)
  •  
  • 13. Panyam J, Zhou WZ, Prabha S, Sahoo SK, Labhasetwar V, Faseb J., 16, 1217 (2002)
  •  
  • 14. Khang G, Lee JH, Lee JW, Cho JC, Lee HB, Korea Polym. J., 8(2), 80 (2000)
  •  
  • 15. Seong H, Moon DS, Khang G, Lee JS, Lee HB, Polym.(Korea), 26(1), 128 (2002)
  •  
  • 16. Panyam J, Zhou WZ, Prabha S, Sahoo SK, Labhasetwar V, Faseb J., 16, 1217 (2002)
  •  
  • 17. Igartua M, Hernandez RM, Esquisabel A, Gascon AR, Calvo MB, Pedraz JL, J. Microencapsul., 14(3), 349 (1997)
  •  
  • 18. Ignatius AA, Claes LE, Biomaterials, 260, 920 (1993)
  •  
  • 19. Hrkach JS, Peracchia MT, Domb A, Lotan N, Langer R, Biomaterials, 18, 27 (1997)
  •  
  • 20. Penco M, Marchioni S, Ferruti P, D'antone S, Deghenghi P, Biomaterials, 17, 1583 (1996)
  •  
  • 21. Lee CW, Bae KS, Polym.(Korea), 26(5), 582 (2002)
  •  
  • 22. Coombes AG, Yeh MK, Lavelle EC, Davis SS, J. Control. Release, 52, 311 (1998)
  •  
  • 23. Seo KS, Park CS, Kim MS, Cho SH, Lee HB, Khang G, Polym.(Korea), 28(3), 211 (2004)
  •  
  • 24. Strock M, Orend KH, Schmitzrixen T, Vasc. Surg., 27, 413 (1993)
  •  
  • 25. Merrill EW, Salzman EW, J. Am. Soc. Artif. Intern. Organs., 6, 60 (1983)
  •  
  • 26. Khang G, Seo SA, Choi HS, Rhee JM, Lee HB, Macromol. Res., 10(5), 246 (2002)
  •  
  • 27. Lee JS, Chae GS, An TK, Khang G, Cho SH, Lee HB, Macromol. Res., 11(3), 183 (2003)
  •  
  • 28. Khang G, Rhee JM, Jeong JK, Lee JS, Kim MS, Cho SH, Lee HB, Macromol. Res., 11(4), 207 (2003)
  •  
  • 29. Khang G, Jeon EK, Rhee JM, Lee I, Lee SJ, Lee HB, Macromol. Res., 11(5), 334 (2003)
  •  
  • 30. Khang G, Jeon JH, Cho JC, Rhee JM, Lee HB, Polym.(Korea), 23(6), 861 (1999)
  •  
  • 31. Khang G, Lee SJ, Jeon JH, Lee JH, Lee HB, Polym.(Korea), 24(6), 869 (2000)
  •  
  • 32. Khang G, Rhee JM, Shin P, Kim IY, Lee B, Lee SJ, Lee YM, Lee HB, Lee I, Macromol. Res., 10(3), 158 (2002)
  •  
  • 33. Seo SA, Choi HS, Lee DH, Khang G, Lee HB, Polym.(Korea), 25(6), 884 (2001)
  •  
  • 34. Jung TK, Oh YM, Shin BC, Polym.(Korea), 27(4), 370 (2003)
  •  
  • 35. Deng X, Zhou S, Li X, Zhao J, Yuan M, J. Control. Release, 71, 165 (2001)
  •  
  • 36. Mallarde D, Boutignon F, Moine F, Barre E, David S, Touchet H, Ferruti P, Dehgenghi R, Int. J. Pharm., 261, 69 (2003)
  •  
  • 37. Bittner B, Witt C, Mader K, Kissel T, J. Control. Release, 60, 297 (1999)
  •  
  • 38. Wan J, Yang Y, Chung T, Tan D, Ng S, Heller J, J. Control. Release, 75, 129 (2001)
  •  
  • 39. Kang J, Schwendeman SP, Biomaterials, 23, 239 (2002)
  •  
  • 40. Jiang W, Schwendeman SP, Pharm. Res., 18, 878 (2001)
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2004; 28(4): 328-334

    Published online Jul 25, 2004

  • Received on Jun 4, 2004
  • Accepted on Jul 21, 2004