Article
  • Fabrication and Characterization of 3D Printed PGA Scaffolds Immobilized with BMP-2 for Bone Tissue Engineering

  • Jiyoung Lee# , Yunjeh Ko# , Ho Yun Chung*, and Oh Hyeong Kwon†

  • Department of Polymer Science and Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, Korea
    *Department of Plastic and Reconstructive Surgery, CMRI, Kyungpook National University School of Medicine, Daegu 41944, Korea

  • 골조직공학을 위한 BMP-2로 고정화된 3D 프린팅 PGA 지지체의 제조 및 특성평가

  • 이지영# · 고윤제# · 정호윤* · 권오형†

  • 국립금오공과대학교 고분자공학과, *경북대학교 의과대학 성형외과, 세포기질연구소

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

References
  • 1. Langer, R.; Vacanti, J. P. Tissue Engineering. Science 1993, 260, 920-926.
  •  
  • 2. Hollister, S. J. Porous Scaffold Design for Tissue Engineering. Nat. Mater. 2006, 4, 518-524.
  •  
  • 3. Hubbell, J. A. Biomaterials in Tissue Engineering. Nat. Biotechnol. 1995, 13, 565-576.
  •  
  • 4. Elanine, N. M.; Patricia, B. W.; Jon, B. M. HumanAnatomy and Physiology, 8th ed.; Benjamin/Cummings Pub. Co: California, 1989.
  •  
  • 5. Bovin, G.; Menuier, P. J. The Mineralization of Bone Tissue: a Forgotten Dimension. Osteoporos. Int. 2003,14, 19-24.
  •  
  • 6. Currey, J. D. Role of Collagen and Other Organics in the Mechanical Properties of Bone, Osteoporos. Int. 2003, 14, 29-36.
  •  
  • 7. Gundbert, C. M. Matrix Proteins, Osteoporos. Int. 2003, 14, 37-42.
  •  
  • 8. Knott, L.; Bailey, A. J. Collagen Cross-links in Mineralizaing Tissues: a Review of Their Chemistry, Function and Clinical Relevance. Bone, 1998, 22, 181-187.
  •  
  • 9. Peter, A.; Ulrich, S.; Astrid, L.; Lutz, C. Mechanics and Mechano-Biology of Fracture Healing in Normal and Ostoeporotic Bone, Osteoporos. Int. 2005, 6, 36-43.
  •  
  • 10. Thomas, P. R.; Richard, E. B.; Christopher, G. M. AO Principles of Fracture Management, 2nd ed.; Thieme: New York, 2000.
  •  
  • 11. Rathner, B. D.; Hoffman, A. S.; Scheon, F. J.; Lemons, J. E. Biomaterals: Principles and Applications, Academic Press, 37-49, 1996.
  •  
  • 12. Hench, L. L.; Clark, A. E.; Schaake, H. F. Effects of Microstructure on the Radiation Stability of Amorphos Semiconductors, J. Non Cryst. Solids 1972, 8, 837-843.
  •  
  • 13. Finkemeier, Christopher G. Bone grafts and bone substitutes, The J. Bone. Joint Surgery, 2002, 84, 454-464.
  •  
  • 14. Kuboki, Y.; Takita, H.; Kobayashi, D.; Tsuruga, E.; Inoue, M.; Murata, N. N.; Dohi, Y.; Ohgushi, H. BMP-induced Osteogenesis on the Surface of Hydroxyapatite with Geometrically Feasible and Nonfeasible Structure: Topology of Osteogenesis, J. Biomedical Mater. Res. 1998, 39, 90-99.
  •  
  • 15. Kim, T.-H.; Kim, D.-E.; Sung, A.-Y. Study on the Functionality and Application of Natural-polymer with Biocompatibility, J. Korean Chem. Soc. 2009,53, 547-553.
  •  
  • 16. Bret, D. U.; Lakshmi, S. N.; Cato, T. L. Biomedical Applications of Biodegradable Polymers, J. Polym. Sci. Part B: Polym. Phys. 2011, 49, 832-864.
  •  
  • 17. Vineet, S.; Meena, T. Structure-Processing-Property Relationship of Poly(Glycolic Acid) for Drug Delivery Systems 1: Synthesis and Catalysis, Int. J. Polym. Sci. 2010, 1-23.
  •  
  • 18. Yeo, T.; Ko, Y.-G.; Kim, E. J.; Kwon, O. K.; Chung, H. Y.; Kwon, O. H. Promoting Bone Regeneration by 3D-printed Poly(glycolic acid)/hydroxyapatite Composite Scaffolds, J. Industrial Eng. Chem. 2021, 94, 343-351.
  •  
  • 19. Lee, W.-K. Development and Application of Hard Tissue Substitutes, KIC News, 2015, 18(4), 25-32.
  •  
  • 20. Yun, Y.-R.; Kim, H.-W.; Jang, J.-H. Application of Growth Factors in Tissue generation, Biomaterals Research, 2013, 14, 133-137.
  •  
  • 21. Elisa, M.; Anne, V.; Catherine, P. Elisabetta Ada Cavalcanti-Adam. Tuning cellular responses to BMP-2 with material surfaces, Cytokine Growth Factor Rev. 2016, 27,43-54.
  •  
  • 22. Jeong, S.-Y.; Chang, W.-T.; Chang, Y.-S.; Ahn, M.-H.; Kim, J.-R.; Song, I.-H. Bone Formation by rhBMP-7 Transduced HEK 293 Cells in Nude Mouse, Yeungnam Univ. J. Med. 2003, 20, 142-151.
  •  
  • 23. Tsuji, K.; Bandyopadhyay, A.; Harfe, B. D.; Coc, K.; Kakar, S.; Gerstenfeld, L.; Einhorm, T.; Tabin, C. J.; Rosen, V. BMP2 Activity, Although Dispensable for Bone Formation, is Required for the Initiation of Fracture Healing, Nature Genetics, 2006, 38, 1424-1429.
  •  
  • 24. Wang, Z.; Wang, Z.; Lu, W. W.; Zhen, W.; Yang, D.; Peng, S. Novel Biomaterial Strategies for Controlled Growth Factor Delivery for Biomedical Applications, NPG Asia Materials, 2017, 9, 1-17.
  •  
  • 25. Kim, E.-H.; Jeong, J.-H.; Han, G.-D.; Son, T.-I. Properties of Photo-Reactive Natural Polymer Derivatives and Its Applications, KIC News, 2015, 18(4), 1-9.
  •  
  • 26. Lin, Q.; Gourdon, D.; Sun, C.; Holten-Anderson, N.; Anderson, T. H.; Waite, J. H.; Israeleachvili, J. N. Adhesion Mechanisms of the Mussel Foot Proteins mfp-1 and mfp-3, Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 3782-3786.
  •  
  • 27. Lee, H.; Dellatore, S. M.; Miller, W. M.; Messersmith, P. B. Mussel-Inspored Surface Chemistry for Multifuntional Coatings, PMC, 2007, 318, 426-430.
  •  
  • 28. Kang, S. M.; Park, S.; Kim, D.; Park, S. Y.; Ruoff, R. S.; Lee, H. Simultaneous Reduction and Surface Functionalization of Graphene Oxide by Mussel-Inspired Chemistry, Adv. Funct. Mater. 2011, 21, 108-112.
  •  
  • 29. Kim, J.; Park, E.; Kim, S.-Y.; Shin, J.-W.; Cho, D.-W. Fabrication of a SFF-based Three-dimensional Scaffold Using a Precision Deposition System in Tissue Engineering, J. Micromech. Microeng. 2008, 18, 27-55.
  •  
  • 30. Gross, B. C.; Lockwood, J. L.; Chen, C.; Spence, D. M. Evaluation of 3D Printing and Its Potential Impact on Biotechnology and the Chemical Sciences, Anal. Chem. 2014, 86, 3240-3252.
  •  
  • 31. Lee, S. J.; Lee, D.; Yoon, T. R. Kim, H. K.; Jo, H. H.; Park, J. S.; Lee, J. H.; Kim, W. D.; Kwon, I. K.; Park, S. A. Surface Modification of 3D-printed Porous Scaffolds via Mussel-inspired Polydopamine and Effective Immobilization of rhBMP-2 to Promote Osteogenic Diffentiation for Bone Tissue Engineering, Acta Biomaterialia, 2016, 40, 182-191.
  •  
  • 32. Jeong, J.-H.; Kim, E.-H.; Han, G.-D.; Nah, J.-W.; Ito, Y. BMP-2 Immobilization by Phosphonated UV-curable Low-molecular-weight Chitosan Derivative on the Surface of Titanium, J. Industrial Eng. Chem. 2016, 34, 33-40.
  •  
  • 33. Cho, S. W. The Introduction of X-ray Spectroscopy for Surface and Interface Electronic Structures, Vacuum Magazine, 2014, 1, 17-20.
  •  
  • 34. Kapuscinski, J. DAPI: a DNA-specific Fluorescent Porbe, Biotech. Histochem. 1995, 70. 220-233.
  •  
  • 35. Russel, W. C.; Newman, C.; Willamson, D. H. A Simple Cytochemical Technique for Demonstration of DNA in Cells Infected with Mycoplasmas and Viruses, Nature, 1975, 36, 461-462.
  •  
  • 36. Park, B.-W.; Choi, M.-J.; Hah, Y.-S.; Cho, H.-Y.; Kim, D. R.; Kim, U.-K.; Kang, H.-J.; Kim, J.-R.; Byun, J.-H. Evalutaion of Osteogenic Activity of Periosteal-derived Cells Treated with Inflammatory Cytokines, J. Korean Assoc. Oral Maxillofac. Surg. 2010, 36, 341-345.
  •  
  • 37. Ciara, M. M.; Matthew, G. H.; Fergal, J. O. The Effect of Meas Pore Size on Cell Attachment, Proliferation and Migration in Collagen-glycosaminoglycan Scaffolds for Boe Tissue Engineering, Biomaterals, 2010, 31, 461-466.
  •  
  • 38. Levengood, S. K. L.; Polak, S. J.; Wheeler, M. B.; Maki, A. J.; Clark, S. G.; Jamison, R. D.; Johnson, A. J. W. Multiscale Osteointegration as a New Paradigm for the Design of Calcium Phosphate Scaffolds for Bone Regeneration, Biomaterials, 2010, 31, 3552-3562.
  •  
  • 39. Salazar, P.; Martín, M.; González-Mora, J. L. Polydopamine-Modified Surfaces in Biosensor Application, In Polymer science: Research Advances, Practical Applications and Educational Aspects, Vilas A. M., Martín, A. S. Eds.; Formatex Research Center S.L.: Badajoz, 2016; pp 385-396.
  •  
  • 40. Swartjes, J. J. T. M.; Sharma, P. K.; van Kooten, T. G.; van der Mei, H. C.; Mahmoudi, M.; Busscher, H. J.; Rochford, E. T. J. Current Developments in Antimicrobial Surface Coatings for Biomedicla Applications, Current Medical Chem. 2014, 22, 2116-2129.
  •  
  • 41. Hong, S.; You, I.; Song, I. T.; Lee, H. Material-Independent Surface Functionalization Inspired byy Mussel-Adhension, Polym. Sci. Technol. 2012, 23, 396-406.
  •  
  • 42. Kister, G.; Cassanas, G. Morphology of Poly(glycolic aicd)by IR and Raman Spectroscopies, Spectrochim. Acta A: Mol. Biomol. Spectrosc. 1997, 53, 1399-1403.
  •  
  • 43. Park, S.-H.; Seo, S.-Y.; Kang, J.-H.; Ito, Y.; Son, T.-I. Preparation of Photocured Azidophenyl-fish Gelatin and Its Capturing of Human Epidermal Growth Factor in Titanium Plate, J. Appl. Polym. Sci. 2013, 127, 154-160.
  •  
  • 44. Seo, S.-Y.; Park, S.-H.; Lee H. J.; Heo, Y.; Na, H. N.; Kim, K. I.; Han, J. H.; Ito Y.; Son, T. I. Coating of Titanium Plate by Photocurable Azidophenyl Chitosn Derivative for Application to Implants, J. Appl. Polym. Sci. 2013, 128, 4322-4326.
  •  
  • 45. Gilde, F.; Maniti, O.; Gullot, R.; Mano, J. F.; Logeart-Acramoglou, D.; Sailhan, F.; Picart, C. Secondary Structure of rhBMP-2 in Protective Biopolymeric Carrier Material, Biomacromolecules, 2012, 13, 3620-3626.
  •  
  • 46. Chung, K. H.; Park, S. H.; Kim, M. K.; Park, H. D.; Son, T. I. Stabilization of Epidermal Growth Factor on Thermal and Proteolytic Degradation by Conjugating with Low Molecular Weight Chitosan, J. Appl. Polym. Sci. 2006, 102, 5072-5082.
  •  
  • 47. Chen, G.; Ito, Y.; Imanishi, Y., Photo-immobilization of Epidermal Growth Factor Enhances its Mitogenic Effect by Artificial Juxtacrine Signaling, Biochim. Biophys. Acta-Mol. Cell Res. 1997, 1358, 200-208.
  •  
  • 48. Woo, Y. K.; Kwon, S. Y.; Lee, H. S.; Park, Y. S. Proliferation of Anterior Cruciate Ligaments Cells in vitro by Photo-immobilized Epidermal Growth Factor, J. Orthopaedic Res. 2007. 25, 73-80.
  •  
  • 49. Huh, J. B.; Kim, S. E.; Kim, H. E.; Kang, S. S.; Choi, K. H.; Jeong, C. M.; Lee, J. Y.; Shin, S. W. Effects of Anodized Implants Coated with Escherichia Coli-derived rhBMP-2 in Beagle Dogs, Int. J. Oral Macillofacial Surgery, 2012, 41, 1577-1584.
  •  
  • 50. Kim, S. E.; Song, S. H.; Yun, Y. P.; Choi, B. J.; Kwon, I. K.; Bae, M. S.; Moon, H. J.; Kwon, Y. D. The Effect of Immobilization of Heparin and Bone Morphogenic Protein-2 (BMP-2) to Titanium Surfaces on Inflammation and Osteoblast Function, Biomaterials, 2011, 32, 366-73.
  •  
  • 51. Lee, S.-H.; Jo, J.-Y.; Yun, M.-J.; Jeon, Y.-C.; Huh, J.-B.; Jeong, C.-M. Effect of Immobilization of the Recombinant Human Bone Morphogenetic Protein 2 (rhBMP-2) on Anodized Implants Coated with Heparin for Improving Alveolar Ridge Augmentation in Beagle Dogs: Radiographic Obsevations, J. Korean Acad. Prosthodont. 2013, 51, 307-314.
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2024; 48(4): 426-439

    Published online Jul 25, 2024

  • 10.7317/pk.2024.48.4.426
  • Received on Apr 15, 2024
  • Revised on Apr 22, 2024
  • Accepted on Apr 23, 2024

Correspondence to

  • Oh Hyeong Kwon

  • Department of Polymer Science and Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, Korea

  • E-mail: ohkwon@kumoh.ac.kr