Article
  • Preparation and Gene Silencing Efficiency of Chitosan-based siRNA Nano-carriers
  • Eunsun Lee*, **, Wheemoon Cho*, Jae Ho Lee***, Sang Jun Park*, Yong-Hee Kim**, and Chun-Ho Kim*,†

  • *Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, 75, Nowon-gil, Nowon-gu, Seoul 01812, Korea
    **Department of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul 04763, Korea
    ***Research Institute for Molecular-Targeted Drugs, Konkuk Institute of Technology, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea

  • 키토산 기반 siRNA 나노전달체 제조 및 유전자 억제 효율
  • 이은선*, ** · 조휘문* · 이재호*** · 박상준* · 김용희** · 김천호*,†

  • *한국원자력의학원 방사선의학연구소, **한양대학교 공과대학 생명공학과, ***건국대학교 질병분자표적신약연구소

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

References
  • 1. Dehousse, V.; Garbacki, N.; Jaspart, S.; Castagne, D.; Piel, G.; Colige, A.; Evrard, B. Comparison of Chitosan/siRNA and Trimethylchitosan/siRNA Complexes Behaviour in vitro. Int. J. Biol. Macromol. 2010, 46, 342-349.
  •  
  • 2. Han, J. H.; Han, S.; Jeong, I. S.; Cheon, S. H.; Kim, S. W. Minicircle‐based GCP‐2 ex vivo Gene Therapy Enhanced the Reepithelialization and Angiogenic Capacity. J. Tissue Eng. Regen. M. 2020, 14, 829-839.
  •  
  • 3. Lee, J. E.; Lee, K.; Nam, J. A.; Kim, A.; Lee, S. Y.; Lee, M. S.; Kim, N. W.; Yin, Y.; Park, J. W.; Park, S. Y. Cellular Delivery of siRNA Using Poly(2-dimethylaminoethyl methacrylate)-Function- alized Graphene Oxide Nano-Wrap. Macromol. Res. 2018, 26, 1115-1122.
  •  
  • 4. Jayakumar, R.; Chennazhi, K.; Muzzarelli, R.; Tamura, H.; Nair, S.; Selvamurugan, N. Chitosan Conjugated DNA Nanoparticles in Gene Therapy. Carbohyd. Polym. 2010, 79, 1-8.
  •  
  • 5. Hoang, Q. T.; Heo, T.-Y.; Choi, D. G.; Choi, S.-H.; Shim, M. S. Guanidinium-Incorporated Micelleplexes for Low Toxic and Efficient siRNA Delivery. Macromol. Res. 2020, 28, 1160-1165.
  •  
  • 6. Ahn, H.; Park, J.-H. Liposomal Delivery Systems for Intestinal Lymphatic Drug Transport. Biomaterials Res. 2016, 20, 36.
  •  
  • 7. Sung, Y. K.; Kim, S. W. Recent Advances in Polymeric Drug Delivery Systems. Biomaterials Res. 2020, 24, 12.
  •  
  • 8. Qin, Y.; Li, P.; Guo, Z. Cationic Chitosan Derivatives as Potential Antifungals: A Review of Structural Optimization and Applications. Carbohyd. Polym. 2020, 236, 116002.
  •  
  • 9. Lee, E.; Park, H.-C.; Lee, D.; Park, S. J.; Kim, Y.-H.; Kim, C.-H. Synthesis and Cellular Affinity of a Water-soluble Sulfated Diselenide Compound as a H2O2-responsive Ionic Cross-linker. J. Ind. Eng. Chem. 2020, 83, 181-188.
  •  
  • 10. Chenthamara, D.; Subramaniam, S.; Ramakrishnan, S. G.; Krishnaswamy, S.; Essa, M. M.; Lin, F.-H.; Qoronfleh, M. W. Therapeutic Efficacy of Nanoparticles and Routes of Administration. Biomaterials Res. 2019, 23, 20.
  •  
  • 11. Plianwong, S.; Opanasopit, P.; Ngawhirunpat, T.; Rojanarata, T., Chitosan Combined with Poly-L-arginine as Efficient, Safe, and Serum-insensitive Vehicle with RNase Protection Ability for siRNA Delivery. Biomed. Res. Int. 2013, 2013, 574136.
  •  
  • 12. Perche, F.; Biswas, S.; Wang, T.; Zhu, L.; Torchilin, V. Hypoxia ‐targeted siRNA Delivery. Angew. Chem. 2014, 126, 3430-3434.
  •  
  • 13. Shen, L.; Li, Y.; Lu, Q.; Qi, X.; Wu, X.; Zhou, Z.; Shen, J. Directed Arrangement of siRNA via Polymerization-induced Electrostatic Self-assembly. Chem. Commun. 2020, 56, 2411-2414.
  •  
  • 14. Jung, B. O.; Kim, C. H.; Choi, K. S.; Lee, Y. M.; Kim, J. J. Preparation of Amphiphilic Chitosan and Their Antimicrobial Activities. J. Appl. Polym. Sci. 1999, 72, 1713-1719.
  •  
  • 15. Kim, C. H.; Choi, K. S. Synthesis and Antibacterial Activity of Quaternized Chitosan Derivatives Having Different Methylene Spacers. J. Ind. Eng. Chem. 2002, 8, 71-76.
  •  
  • 16. Yang, H. M.; Won, Y. H.; Yoon, H. Y.; Kim, C. H.; Goo, Y. T.; Chang, I. H.; Choi, Y. W. Screening of Polymer Additives in Poloxamer 407 Hydrogel Formulations for Intravesical Instillation: Evaluation of Mechanical Properties, Gel-forming Capacity, and Drug Release. Polym. Korea 2020, 44, 817-826.
  •  
  • 17. Sun, M. T.; O’Connor, A. J.; Milne, I.; Biswas, D.; Casson, R.; Wood, J.; Selva, D. Development of Macroporous Chitosan Scaffolds for Eyelid Tarsus Tissue Engineering. Tissue Eng. Regen. Med. 2019, 16, 595-604.
  •  
  • 18. Kim, C. H.; Choi, J. W.; Chun, H. J.; Choi, K. S. Synthesis of Chitosan Derivatives with Quaternary Ammonium Salt and Their Antibacterial Activity. Polym. Bull. 1997, 38, 387-393.
  •  
  • 19. Gu, B. K.; Park, S. J.; Kim, M. S.; Kang, C. M.; Kim, J.-I.; Kim, C.-H. Fabrication of Sonicated Chitosan Nanofiber Mat with Enlarged Porosity for Use as Hemostatic Materials. Carbohyd. Polym. 2013, 97, 65-73.
  •  
  • 20. Park, S. J.; Cho, W.; Kim, M. S.; Gu, B. K.; Kang, C. M.; Khang, G.; Kim, C. H. Substance‐P and Transforming Growth Factor‐β in Chitosan Microparticle‐pluronic Hydrogel Accelerates Regenerative Wound Repair of Skin Injury by Local Ionizing Radiation. J. Tissue Eng. Regen. M. 2018, 12, 890-896.
  •  
  • 21. Abarca-Cabrera, L.; Fraga-García, P.; Berensmeier, S. Bio-nano Interactions: Binding Proteins, Polysaccharides, Lipids and Nucleic Acids onto Magnetic Nanoparticles. Biomater. Res. 2021, 25, 1-18.
  •  
  • 22. Mou, D.; Yu, Q.; Zhang, J.; Zhou, J.; Li, X.; Zhuang, W.; Yang, X. Intra-articular Injection of Chitosan-Based Supramolecular Hydrogel for Osteoarthritis Treatment. Tissue Eng. Regen. Med. 2021, 18, 113-125.
  •  
  • 23. Wang, J. J.; Zeng, Z. W.; Xiao, R. Z.; Xie, T.; Zhou, G. L.; Zhan, X. R.; Wang, S. L. Recent Advances of Chitosan Nanoparticles as Drug Carriers. Int. J. Nanomed. 2011, 6, 765.
  •  
  • 24. Ragelle, H.; Riva, R.; Vandermeulen, G.; Naeye, B.; Pourcelle, V.; Le Duff, C. S.; D'Haese, C.; Nysten, B.; Braeckmans, K.; De Smedt, S. C. Chitosan Nanoparticles for siRNA Delivery: Optimizing Formulation to Increase Stability and Efficiency. J. Contol. Release 2014, 176, 54-63.
  •  
  • 25. Kim, C.-H.; Park, S. J.; Yang, D. H.; Chun, H. J. Chitosan for Tissue Engineering. Novel Biomed. Regen. Med. 2018, 475-485.
  •  
  • 26. Jun, G. Y.; Ko, Y.-G.; Kim, D.; Kwon, O. K.; Kim, W. I.; Chung, H. Y.; Kwon, O. H. Characterization of Chitosan-Poly(epsilon-caprolactone) Hybrid Nanofiber Sheets Containing Insulin for Wound Dressings. Polym. Korea 2020, 44, 549-558.
  •  
  • 27. Liao, Z.-X.; Ho, Y.-C.; Chen, H.-L.; Peng, S.-F.; Hsiao, C.-W.; Sung, H.-W. Enhancement of Efficiencies of the Cellular Uptake and Gene Silencing of Chitosan/siRNA Complexes via the Inclusion of a Negatively Charged Poly(γ-glutamic acid). Biomaterials 2010, 31, 8780-8788.
  •  
  • 28. Mao, S.; Sun, W.; Kissel, T. Chitosan-based Formulations for Delivery of DNA and siRNA. Adv. Drug Deliver. Rev. 2010, 62, 12-27.
  •  
  • 29. Hong, W.-G.; Nah, J.-W.; Jeong, G.-W. Preparation and Characterization of Tropolone-encapsulated Chitosan Nanoparticle for Application as Cosmetic Preservatives. Polym. Korea 2020, 44, 535-541.
  •  
  • 30. Yang, D. H.; Seo, D. I.; Lee, D.-W.; Bhang, S. H.; Park, K.; Jang, G.; Kim, C. H.; Chun, H. J. Preparation and Evaluation of Visible-light Cured Glycol Chitosan Hydrogel Dressing Containing Dual Growth Factors for Accelerated Wound Healing. J. Ind. Eng. Chem. 2017, 53, 360-370.
  •  
  • 31. Jeong, S. Y.; Kim, E.; Park, W. H. Gelation Behavior and Self-healing Property of Glycol Chitosan with Catechol Functionality. Polym. Korea 2020, 44, 225-232.
  •  
  • 32. Kim, C. H.; Chun, H. J. A synthesis of O-diethylaminoethyl Chitosan and its Binding Ability of Cholate and Deoxycholate Anion in vitro. Polym. Bull. 1999, 42, 25-32.
  •  
  • 33. Salimifard, S.; Kiani, F. K.; Eshaghi, F. S.; Izadi, S.; Shahdadnejad, K.; Masjedi, A.; Heydari, M.; Ahmadi, A.; Hojjat-Farsangi, M.; Hassannia, H. Codelivery of BV6 and anti-IL6 siRNA by Hyaluronate-conjugated PEG-chitosan-lactate Nano- particles Inhibits Tumor Progression. Life Sci. 2020, 260, 118423.
  •  
  • 34. Katas, H.; Alpar, H. O. Development and Characterisation of Chitosan Nanoparticles for siRNA Delivery. J. Contol. Release 2006, 115, 216-225.
  •  
  • 35. Raja, M. A. G.; Katas, H.; Wen, T. J. Stability, Intracellular Delivery, and Release of siRNA from Chitosan Nanoparticles Using Different Cross-linkers. PloS ONE 2015, 10, e0128963.
  •  
  • 36. Li, Y.; Upadhyay, S.; Bhuiyan, M.; Sarkar, F. H. Induction of Apoptosis in Breast Cancer Cells MDA-MB-231 by Genistein. Oncogene 1999, 18, 3166-3172.
  •  
  • 37. Zhou, K.; Xie, L.; Peng, X.; Guo, Q.; Wu, Q.; Wang, W.; Zhang, G.; Wu, J.; Zhang, G.; Du, C. CXCR4 Antagonist AMD3100 Enhances the Response of MDA-MB-231 Triple-negative Breast Cancer Cells to Ionizing Radiation. Cancer Lett. 2018, 418, 196-203.
  •  
  • 38. Lallana, E.; Rios de la Rosa, J. M.; Tirella, A.; Pelliccia, M.; Gennari, A.; Stratford, I. J.; Puri, S.; Ashford, M.; Tirelli, N. Chitosan/hyaluronic Acid Nanoparticles: Rational Design Revisited for RNA Delivery. Mol. Phrm. 2017, 14, 2422-2436.
  •  
  • 39. Ragelle, H.; Vanvarenberg, K.; Vandermeulen, G.; Préat, V. Chitosan Nanoparticles for SiRNA Delivery in vitro. In SiRNA Delivery Methods; Shum, K., Rossi, J., Eds.; Springer: New York, 2016; pp 143-150.
  •  
  • 40. Songsurang, K.; Praphairaksit, N.; Siraleartmukul, K.; Muangsin, N. Electrospray Fabrication of Doxorubicin-chitosan-tripoly- phosphate Nanoparticles for Delivery of Doxorubicin. Arch. Pharm. Res. 2011, 34, 583.
  •  
  • 41. Palmeira-de-Oliveira, R.; Palmeira-de-Oliveira, A.; Gaspar, C.; Silvestre, S.; Martinez-de-Oliveira, J.; Amaral, M.; Breitenfeld, L. Sodium Tripolyphosphate: An Excipient with Intrinsic in vitro anti-Candida Activity. Int. J. Pharm. 2011, 421, 130-134.
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  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2021; 45(5): 697-703

    Published online Sep 25, 2021

  • 10.7317/pk.2021.45.5.697
  • Received on Mar 12, 2021
  • Revised on Jun 7, 2021
  • Accepted on Jun 24, 2021

Correspondence to

  • Chun-Ho Kim*
  • *Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, 75, Nowon-gil, Nowon-gu, Seoul 01812, Korea

  • E-mail: chkim@kirams.re.kr