Article
  • Fabrication and Characterization of 3D Printed PGA Scaffolds Immobilized with BMP-2 for Bone Tissue Engineering

  • Jiyoung Lee# , Yunjeh Ko# , Ho Yun Chung*, and Oh Hyeong Kwon†

  • Department of Polymer Science and Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, Korea
    *Department of Plastic and Reconstructive Surgery, CMRI, Kyungpook National University School of Medicine, Daegu 41944, Korea

  • 골조직공학을 위한 BMP-2로 고정화된 3D 프린팅 PGA 지지체의 제조 및 특성평가

  • 이지영# · 고윤제# · 정호윤* · 권오형†

  • 국립금오공과대학교 고분자공학과, *경북대학교 의과대학 성형외과, 세포기질연구소

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

Abstract

This study focused on immobilizing bone morphogenetic protein-2 (BMP-2) onto a 3D-printed poly(glycolic acid) (PGA) scaffold to develop a biodegradable scaffold for continuous bone formation stimulation in bone tissue engineering. BMP-2 immobilization was achieved using the UV irradiation method and confirmed through ATR-FTIR and XPS analyses. The scaffold's structure, hydrolytic degradation behavior, and in vitro cell viability were analyzed. Furthermore, the BMP-2 release behavior and calcification performance of the scaffold were evaluated for osteogenic tissue engineering applications. Results from in vivo animal model experiments and histological analyses demonstrated that the BMP-2 immobilized PGA scaffold exhibited superior bone tissue regeneration ability compared to the control group. And, It suggests potential as a scaffold in bone tissue engineering.


본 연구에서는 골형성 촉진인자의 고정화를 통하여 지속적인 자극이 가능한 생분해성 골조직공학용 지지체를 개발하고자 3D 프린팅된 폴리글리콜산(PGA) 지지체에 bone morphogenetic protein-2(BMP-2)를 고정하여 성능을 검증하였다. BMP-2 고정화는 UV 조사법을 이용하였으며, 개질여부는 ATR-FTIR 및 XPS를 통해 확인하였다. 지지체의 구조, 가수분해 거동, in vitro 세포생존율을 분석하였으며, 골형성 조직공학용 지지체로서 BMP-2 방출거동, calcification 성능을 평가하였다. In vivo 동물모델실험 및 조직학적 평가결과에서 BMP-2가 고정된 PGA 지지체는 대조군에 비하여 우수한 골조직 재생능을 보여주었으며, 골조직공학용 지지체로서의 활용이 기대된다.


Keywords: poly(glycolic acid), scaffold, bone morphogenetic protein-2, 3D printing, bone tissue engineering.

  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2024; 48(4): 426-439

    Published online Jul 25, 2024

  • 10.7317/pk.2024.48.4.426
  • Received on Apr 15, 2024
  • Revised on Apr 22, 2024
  • Accepted on Apr 23, 2024

Correspondence to

  • Oh Hyeong Kwon

  • Department of Polymer Science and Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, Korea

  • E-mail: ohkwon@kumoh.ac.kr