In this study, an alginate hydrogel was prepared and characterized through an enzymatic crosslinking reaction of modified alginate with tyramine (Alg-Tyr) in order to develop an injectable wound healing agent that secures a moisture environment and dense adhesion to rough wound surface with controlled physical properties. The modification of Alg-Tyr was confirmed through attenuated total reflection-Fourier transform infrared spectroscope and proton nuclear magnetic resonance spectrometer. The storage modulus, gelation time, hydrolysis behavior and in vitro cytotoxicity of the hydrogel according to the crosslinking and concentration parameters of the alginate mixture were analyzed. The optimum hydrogel was selected as Alg-Tyr 4 w/w%, horseradish peroxidase 0.2 mg/mL and H₂O₂ 4 μL/mL concentration of composition. As results of in vivo wound healing rate and histological evaluation, the alginate hydrogel group showed superior recovery speed and skin regeneration ability compared to the control group, and it is expected to be used as an injectable wound healing agent.

본 연구에서는 굴곡이 심한 창상면에서의 밀착성 및 습윤환경이 보장되고 물성이 제어된 주사형 창상치료제를 개발하고자 티라민이 도입된 알긴산(Alg-Tyr)을 합성한 후, 효소촉매 가교반응을 이용한 알긴산 하이드로젤을 제조하여 성능을 검증하였다. Alg-Tyr의 개질여부는 attenuated total reflection-Fourier transform infrared spectroscope 및 proton nuclear magnetic resonance spectrometer을 통해 확인하였다. 알긴산 혼합물의 가교반응에 관여하는 구성물의 농도 변수에 따른 하이드로젤의 저장탄성률, 젤화시간, 가수분해 거동, in vitro 세포독성을 분석하였으며, 주사형 창상치료제로서 최적조건의 하이드로젤은 Alg-Tyr 4 w/w%, horseradish peroxidase 0.2 mg/mL, H₂O₂ 4 μL/mL 농도의 배합으로 선정하였다. In vivo 창상치유도 및 조직학적 평가결과에서 알긴산 하이드로젤군은 대조군에 비하여 우수한 회복속도 및 피부 재생능을 보여주었으며, 주사형 창상치료제로서의 활용이 기대된다.

Keywords: alginate, hydrogel, injectable, enzymatic reaction, wound healing agent.