Article
  • Gelatin-Chitosan Membrane Containing Fish Bones for Guided Bone Regeneration

  • Ji Sun Park*,#, Jin Kim**,#, Geon Yi Kang***, and Chang-Moon Lee*, ***, ****, *****,†

  • *Interdisciplinary Program of Perfume and Cosmetics, Chonnam National University, Gwangju 61186, Korea
    **Department of Oral and Maxillofacial Surgery, Chosun University Dental Hospital, Gwangju 61452, Korea
    ***Department of Biomedical Engineering, Chonnam National University, Jeonnam, Yeosu 59626, Korea
    ****School of Healthcare and Biomedical Engineering, Chonnam National University, Jeonnam, Yeosu 59626, Korea
    *****Research Center of Healthcare and Biomedical Engineering, Chonnam National University, Jeonnam, Yeosu 59626, Korea

  • 골 유도 재생을 위한 어골 함유 젤라틴-키토산 차폐막

  • 박지선*,# ·김 진**,# ·강건이*** ·이창문*, ***, ****, *****,†

  • *전남대학교 대학원 향장품학협동과정, **조선대학교 치과대학 구강악안면외과, ***전남대학교 의공학과,
    ****전남대학교 헬스케어메디컬공학부, *****전남대학교 헬스케어의공학연구소

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

Abstract

Calcium phosphate obtained from fish bones is expected to be used as a material for bone regeneration. In this study, fish bones-included gelatin-chitosan membrane (FB@GEL-CS membrane) was prepared and investigated for guided bone regeneration (GBR). Cell proliferation of osteoblast MC3T3-E1 on FB@GEL-CS membrane according to the content of fish bones was evaluated. MC3T3-E1 cells grew much better on FB@CS-GEL membrane as the content of fish bones increase. In addition, MC3T3-E1 cells on FB@GEL-CS membrane significantly increased alkaline phosphatase (ALP) activity according to the content of fish bones. Analyses of alizarin-red S staining showed that MC3T3-E1 cells on FB@GEL-CS membrane caused increases in cell maturation as the content of fish bones increase. Taken together, results of this study demonstrate that FB@GEL-CS membrane can induce MC3T3-E1 cell proliferation and maturation.


어골은 골 대사와 연관된 유용한 성분을 포함하고 있어서 골 재생을 위한 재료로 활용이 기대된다. 본 연구에서는 어골을 함유한 젤라틴-키토산 차폐막을 제조하고 골 유도 재생에 있어서 골세포의 증식과 분화에 미치는 영향을 평가하였다. 어골의 함량이 증가함에 따라 젤라틴-키토산 차폐막에서 MC3T3-E1 세포의 증식은 증가하는 경향을 보였다. MC3T3-E1 세포의 alkaline phosphatase(ALP) 활성은 젤라틴-키토산 차폐막에서 어골의 함량이 증가함에 따라 유의하게 증가하였다. Alizarin-red S 염색 시험에서 어골의 함량이 증가함에 따라 젤라틴-키토산 차폐막은 MC3T3-E1 세포의 성숙을 유의하게 유도하는 것으로 나타났다. 따라서, 어골을 함유한 젤라틴-키토산 차폐막은 골세포의 증식과 성숙을 유도할 수 있고 골 유도 재생에 유용하게 사용될 수 있다.


Keywords: fish bone, chitosan, gelatin, guided bone regeneration, osteoblast.

  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2022; 46(6): 849-854

    Published online Nov 25, 2022

  • 10.7317/pk.2022.46.6.849
  • Received on Sep 7, 2022
  • Revised on Sep 14, 2022
  • Accepted on Sep 14, 2022

Correspondence to

  • Chang-Moon Lee

  • *Interdisciplinary Program of Perfume and Cosmetics, Chonnam National University, Gwangju 61186, Korea
    ***Department of Biomedical Engineering, Chonnam National University, Jeonnam, Yeosu 59626, Korea
    ****School of Healthcare and Biomedical Engineering, Chonnam National University, Jeonnam, Yeosu 59626, Korea
    *****Research Center of Healthcare and Biomedical Engineering, Chonnam National University, Jeonnam, Yeosu 59626, Korea

  • E-mail: cmlee@jnu.ac.kr