Article
  • Layer-by-layer Adsorption of Glucose Oxidase on Graphene-polystyrene Composite for Glucose Detection
  • Min Jae Shin

  • Department of Cosmetics and Biotechnology, Semyung University, Jecheon 27136, Korea

  • 글루코오스 감지를 위한 그래핀-폴리스티렌 복합체 위에 층 쌓기 방법에 의한 글루코오스 산화 효소의 흡착
  • 신민재

  • 세명대학교 화장품 생명공학과

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

Abstract

An electrically conductive graphene-polystyrene composite was prepared by mixing an exfoliated phenylisocyanate-treated graphite oxide sheet with polystyrene, followed by chemical reduction with dimethylhydrazine. Glucose oxidase was immobilized on this composite via electrostatic interaction using a layer-by-layer adsorption technique, with poly(diallyldimethylammonium chloride) as the counter-ionic material. A quartz crystal microbalance was used to estimate the amount of the adsorbed layer material. An amperometric glucose biosensor, fabricated with the glucose oxidase-immobilized graphene-polystyrene composite as a working electrode, showed significantly improved efficacy for measuring glucose concentration.


Phenylisocyanate로 처리된 층 분리된 graphite oxide와 폴리스티렌을 혼합하고 dimethylhydrazine으로 환원시켜 전도성을 갖는 그래핀-폴리스티렌 복합체를 제조하였다. 글루코오스 산화 효소는 poly(diallyldimethylammonium chloride)를 반대편 상대 물질로 사용하고, 층 쌓기 흡착 방법을 이용해서 정전기적인 인력으로 이 복합체 위에 고정되었다. 층을 형성하는 물질의 양은 quartz crystal microbalance를 이용해서 측정하였다. 그래핀-폴리스티렌 복합체에 고정된 글루코오스 산화 효소를 이용해서 형성된 전류 측정 방식의 글루코오스 센서는 글루코오스 농도 측정에 상당히 우수한 결과들을 보여 주었다.


Keywords: glucose, biosensor, graphene, composite, electrostatic layer-by-layer adsorption.

  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2022; 46(5): 640-645

    Published online Sep 25, 2022

  • 10.7317/pk.2022.46.5.640
  • Received on May 11, 2022
  • Revised on Jul 18, 2022
  • Accepted on Aug 2, 2022

Correspondence to

  • Min Jae Shin
  • Department of Cosmetics and Biotechnology, Semyung University, Jecheon 27136, Korea

  • E-mail: newminj@gmail.com