Note
  • Polypyrrole/Poly(1-(2-carboxyehtyl)pyrrole) Bilayered Composite Scaffold for Cell Adhesion
  • Joo-Woon Lee

  • Chemistry - School of Liberal Arts and Sciences, Korea National University of Transportation, Chungju, Chungbuk 27469, Korea

  • 이중층 폴리피롤 복합재료 지지체
  • 이주운

  • 한국교통대학교 교양학부-화학

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

Abstract

Polypyrrole/poly(1-(2-carboxyethyl)pyrrole) (PPy/PPyCOOH) bilayered composite is demonstrated as a platform for surface modification and cell attachment. The composite is developed by polymerizing 1-(2-carboxyethyl)pyrrole onto the surface of PPy film using an electrochemical layer-by-layer deposition technique. FTIR and X-ray photoelectron spectroscopy (XPS) are used to determine the presence of carboxylic acid functionality (-COOH) at the PPyCOOH layer surface of the bilayered composite. A four-point probe analysis is used to verify electrical conductivity in the semiconductor range. The carboxylic acid functionality is further tailored by chemically conjugating a cell-adhesive Arg-Gly-Asp (RGD)-containing oligopeptide, GRGDSP, onto the PPyCOOH surface of the composite. Human umbilical vein endothelial cells (HUVECs) cultured on the RGD-grafted composite successfully demonstrate the improved cell adhesion and spreading compared with an ungrafted control PPy/PPyCOOH.


전도성 고분자-폴리피롤은 화학구조상 공유결합을 할 수 있는 작용기가 존재하지 않는다. 이에 상용 1-(2-cyanoethyl)- pyrrole을 가수분해 반응시켜 카복실산 작용기를 갖는 피롤 유도단량체를 합성하고 이를 폴리피롤의 표면상에서 전기화학적으로 고분자 중합하여, 이중층으로 구성된 전도성 복합재료 지지체를 개발하였다. 지지체 표면에 도입한 카복실산 작용기와 조직인식(Arg-Gly-Asp(RGD)를 포함하는)-올리고펩타이드를 선택적으로 화학결합시킨 후, 모델 세포조직-인간 제대혈관 내피세포(HUVEC)의 흡착 및 증식정도에 대한 정성 및 정량평가를 통해 생물학적 활성이 증진됨을 확인할 수 있었다. 따라서, 이상에서 개발된 전도성 폴리피롤 복합재료는 조직공학을 위한 생체모사용 지지체로서 다양한 플랫폼 역할을 할 것으로 기대된다.


Keywords: polypyrrole, conductive composite, scaffold, tissue engineering.

  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2020 Impact Factor : 0.493
  • Indexed in SCIE

This Article

  • 2022; 46(2): 295-299

    Published online Mar 25, 2022

  • 10.7317/pk.2022.46.2.295
  • Received on Dec 5, 2021
  • Revised on Jan 13, 2022
  • Accepted on Jan 16, 2022

Correspondence to

  • Joo-Woon Lee
  • Chemistry - School of Liberal Arts and Sciences, Korea National University of Transportation, Chungju, Chungbuk 27469, Korea

  • E-mail: jwoonlee@ut.ac.kr