One of the significant natural bioactive materials is demineralized bone particle (DBP) whose has a powerful inducer of new bone growth. In this study, we developed the DBP loaded poly-lactide (PLA) and poly(Llactide-co-glycolide) (PLGA) scaffolds for the possibility of the application of the tissue engineered bone. PLA/DBP and PLGA/DBP scaffolds were prepared by solvent casting/salt leaching method and were characterized by porosimeter, scanning electron microscopy. BMSCs were stimulated by osteogenic medium and characterized by histological stained Wright-Giemsa, Alizarin red, von Kossa, and alkaline phosphate activity (ALP). DBP impregnated scaffolds with BMSCs were implanted into the back of athymic nude mouse to observe the effect of DBP on the osteoinduction compared with control scaffolds. It can be observed that the porosity was above 90.2% and the pore size was above 69.1 μm. BMSCs could be differentiated into osteoprogenitor cells as result of wrightgiemsa, alizarin red, von Kossa and ALP staining. In in vivo study, we could observed calcification region in PLA/DBP and PLGA/DBP groups, but calcification did not occur almost in control scaffolds. From these results, it seems that DBP as well as BMSCs play an important role for bone induction in PLA/DBP and PLGA/DBP scaffolds.

생체적합성 천연재료 중 하나인 탈미네랄화된 골분 (demineralized bone particle, DBP)은 골형성단백질 (BMP)을 함유하고 있어 골수간엽줄기세포 (BMSC_s)의 분화를 유도한다. 본 연구에서는 DBP를 함유한 폴리락타이드 (PLA)와 락타이드-글리콜라이드 공중합체 (PLGA) 다공성 지지체를 용매 캐스팅/염추출법으로 제조하였고, 수은다공측정계 및 전자주사현미경을 이용하여 특성결정 하였다. BMSC_s는 골분화 배지를 이용하여 조골세포로 분화시켜 Wright-Giemsa, Alizarin red, von kossa 및 ALP 염색으로 확인하였다. DBP가 함유된 지지체와 DBP가 함유되지 않은 지지체에 BMSC_s를 파종한 후 면역결핍 누드마우스의 피하에 삽입하여 이들의 골형성 정도를 비교하여 보았다. 제조한 지지체의 다공도는 90.2% 이상이었고 평균 다공크기도 69.1 μm 이상이었다. BMSC_s는 Wright-Giemsa, Alizarin red, von kossa 및 ALP 염색결과 조골세포로 분화가 가능했으며, 동물실험을 수행한 결과 DBP가 함유된 지지체에서 칼슘침착 영역을 확인할 수 있었지만 DBP가 함유 되지 않은 지지체에서는 칼슘침착 영역을 확인하지 못하였다. 결론적으로 DBP를 함유한 지지체에서 DBP와 BMSC_s가 골형성에 중요한 요인으로 작용한다고 사료된다.

Keywords: scaffold; DBP; BMSCs; osteogenesis; tissue engineered bone