Article
  • Solid Phase Synthesis of Lysine-exposed Peptide-Polymer Hybrids by Atom Transfer Radical Polymerization
  • Ha EJ, Kim M, Kim J, An SSA, Paik H
  • ATRP를 이용한 Lysine 말단기를 가진 펩타이드-고분자 하이브리드 합성
  • 하은주, 김미진, 김진구, 안성수, 백현종
Abstract
Recently, the peptide(or protein)-polymer hybrid materials (PPs) were sought in many research areas as potential building blocks for assembling nanostructures in selective solvents. In PPs, the facile routes of preparing well-defined peptide-polymer bio-conjugates and their specific activities in various applications are important issues. Our strategy to prepare the peptide-polymer hybrid materials was to combine atom transfer radical polymerization (ATRP) method with solid phase peptide synthesis. The standard solid phase peptide synthesis method was employed to prepare the PYGK(proline-tyrosine-glycine-lysine) peptide. PYGK is an analogue peptide, PFGK (proline-phenylalanine-glycine-lysine), which interacted with plasminogen in fibrinolysis. The peptide and the peptide-initiator were characterized with MALDITOF mass spectrometry and 1H NMR spectrometer. The peptide-polymer, pSt-PYGK was characterized by GPC, IR, 1H NMR spectrometer and TLC. Spherical micellar aggregates were determined by TEM and SEM. Current synthesis methodology suggested opportunities to create the well-defined peptide-polymer hybrid materials with specific binding activity.

펩타이드-고분자 하이브리드 소재(PPs)들은 선택적 용매에서 나노구조 형성을 위한 잠재적 구성 요소로서 많은 연구분야에 이용되고 있다. PPs는 잘 정의된 펩타이드-고분자로 이루어진 바이오콘주게이트의 손쉬운 제조방법과 다양한 응용분야에서 이들의 고유활성도에 대한 연구는 중요한 이슈이다. 본 연구에서는 atom transfer radical polymerization(ATRP)와 고체상 펩타이드 합성법을 이용하여 펩타이드-고분자 하이브리드 소재를 제조하였다. PYGK(proline-tyrosine-glycine-lysine) 펩타이드를 제조하기 위하여 일반적인 고체상 펩타이드 합성법을 이용하였다. PYGK 펩타이드는 섬유소용해(fibrinolysis) 과정에서 플라스미노젠과 반응하는 PFGK(proline-phenylalanine-glycine-lysine)와 유사한 펩타이드이다. 펩타이드와 펩타이드 개시제는 MALDI-TOF와 1H NMR을 이용하여 분석하였다. 펩타이드-고분자인 pSt-PYGK는 GPC, IR, 1H NMR 분석법, 그리고 TLC를 이용하여 분석하였다. 구형 마이셀 집합체는 TEM과 SEM으로 측정하였다. 본 합성방법은 고유결합 활성도를 가진 잘 정의된 펩타이드-고분자 하이브리드 소재를 합성할 수 있는 기회를 제공한다.

Keywords: atom transfer radical polymerization; plasminogen; coagulation; polystyrene; polymer-protein hybrid.

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  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2014; 38(4): 550-556

    Published online Jul 25, 2014

  • Received on Apr 14, 2014
  • Accepted on Jun 5, 2014