Article
  • Regeneration of Intervertebral Disc Using Poly(lactic-co-glycolic acid) Scaffolds Included Demineralized Bone Particle In Vivo
  • Jang JE, Kim HY, Song JE, Lee D, Kwon SY, Chung JW, Khang G
  • In vivo 상에서 탈미네랄화된 골분이 함유된 PLGA 지지체를 이용한 추간판 디스크 재생
  • 장지은, 김혜윤, 송정은, 이동원, 권순용, 정진화, 강길선
Abstract
Demineralized bone particle (DBP) is a biomaterial used widely in the field of tissue engineering. In this study, in order to study the effect of DBP/poly(lactic-co-glycolic acid) (PLGA) scaffold on disc regeneration in vivo environment, we prepared the porous DBP/PLGA hybrid scaffold. Disc defect was induced by removing the nucleus pulposus tissue after incision the annulus fibrosus tissue in half and scaffolds were transplanted. After 1, 2 and 3 months later, the extracted discs were confirmed by collagen synthesis and glycosaminoglycan (sGAG). We conducted histology (H&E, Safranin-O, Alcian blue, Type I Collagen, Type II Collagen). From the results, it was confirmed that collagen and sGAG content were high in DBP/PLGA scaffold, and the regeneration of intervertebral disc was possible.

탈미네랄화된 골분(demineralized bone particle, DBP)은 연골 형성의 유도인자로 사용되기 때문에 조직공학에서 널리 사용되는 생체재료이다. 본 연구에서는 in vivo 환경에서 디스크 재생 효과를 연구하기 위해 DBP를 poly(lactic-co-glycolic acid) (PLGA)에 첨가하여 다공성 지지체를 제조하였다. 디스크의 섬유륜 조직을 반으로 절개한 후, 수핵 조직을 제거하여 디스크 결손을 유발시켰다. 빈 공간에 PLGA, DBP/PLGA 지지체를 이식하여 in vivo 환경에서 조직공학적 디스크 재생을 관찰하였다. 1, 2 및 3개월 후 디스크를 적출하여 글리코스아미노글라이칸 (glycosaminoglycan, sGAG) 및 콜라겐 합성 정도를 측정하였으며 조직학적 평가로 H&E, Safranin-O, Alcian blue 염색과 면역조직학적 평가로 제 I형 콜라겐, 제 II형 콜라겐 염색을 수행하였다. 그 결과 DBP/PLGA 지지체에서 sGAG 및 콜라겐 함량이 높은 것을 확인하였으며 추간판 디스크 재생의 가능성을 확인하였다.

Keywords: demineralized bone particle; poly(lactic-co-glycolic acid); scaffold; disc regeneration.

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  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2013; 37(6): 669-676

    Published online Nov 25, 2013

  • Received on May 6, 2013
  • Accepted on Jun 12, 2013