Article
  • Adhesion of Human Intervertebral Disk Cells on Alginate/PLGA Microspheres
  • Lee JH, Jang JW, So JW, Choi JH, Park JH, Ahn SI, Son YS, Min BH, Khang GS
  • Alginate/PLGA 미립구에 대한 인간디스크 세포 부착 효과
  • 이준희, 장지욱, 소정원, 최진희, 박종학, 안식일, 손영숙, 민병현, 강길선
Abstract
PLGA microspheres have been known as an injectable system for tissue engineering. The purpose of this study was to investigate the condition of emulsion formation and cell adhesion on the microsphere surface. BSA-loaded PLGA microsphere was fabricated by oil-in-water (O/W) and water-in-oil-in-water (W/O/W) solvent evaporation method. Sodium alginate was dissolved in water phase to control initial burst release and to improve lag time by PLGA bulk degradation. In addition, the morphology of cells attached on the microspheres was studied using a scanning electron microscopy (SEM). Cellular proliferation behavior of human disc cells cultivated on PLGA microspheres was analyzed using a MTT assay. MTT assay revealed that the cells can attach and proliferate on PLGA microspheres. According to these results, we concluded that BSA-loaded alginate/PLGA microspheres can be used as an injectable system for tissue engineering application.

PLGA미립구는 주사제형과 같은 형태로 조직공학적 응용에 이용할 수 있다. 본 연구에서는 미립구 제조시 에멀젼 형성조건에 대한 영향과 미립구 표면에 세포를 부착시키는 방법에 대하여 연구하였다. BSA를 함유하는 PLGA미립구는 수중유형(O/W)과 수중유중수형(W/O/W) 용매증발법을 이용하여 제조하였다. 미립구의 초기방출효과제어와 PLGA분해의 과정에서 발생되는 지연시간을 개선시키기 위하여 알긴산나트륨을 수상에 용해시켜 사용하였다. 미립구에 부착된 세포의 형태를 전자주사현미경(SEM)을 이용하여 분석하였고 PLGA미립구에 배양된 인간디스크세포의 증식은 MTT분석을 이용하였으며 이를 통하여 PLGA미립구 표면에 세포가 부착되었음을 확인하였다. 본 연구는 BSA가 함유된 알긴산/PLGA미립구를 이용하여 조직공학적 응용이 가능한 주사제형으로서의 가능성을 제안하였다.

Keywords: alginate; PLGA; microsphere; cell adhesion; initial burst

References
  • 1. Piskin E, J. Biomat. Sci. Polym. Ed., 6, 775 (1995)
  •  
  • 2. Nair LS, Laurencin CT, Pro. Polym. Sci., 32, 762 (2007)
  •  
  • 3. Khang G, Jeon JH, Cho JC, Lee HB, Polym.(Korea), 23(3), 471 (1999)
  •  
  • 4. Choi MG, Khang G, Lee IW, Rhee JM, Lee HB, Polym.(Korea), 25(3), 318 (2001)
  •  
  • 5. Jeon EK, Whang HJ, Khang G, Lee I, Rhee JM, Lee HB, Polym.(Korea), 25(6), 893 (2001)
  •  
  • 6. Park TG, Lee HY, Nam YS, J. Control. Release, 55, 181 (1998)
  •  
  • 7. Choi SH, Park TG, Int. J. Pharm., 203, 193 (2000)
  •  
  • 8. Chung HJ, Park TG, Adv. Drug. Deliver. Rev., 59, 249 (2007)
  •  
  • 9. Bondar B, Fuchs S, Motta A, Migliaresi C, Kirkpatrick CJ, Biomaterials, 29, 561 (2008)
  •  
  • 10. Chew SY, Mi R, Hoke A, Leong KW, Biomaterials, 29, 653 (2008)
  •  
  • 11. Lee SJ, Oh SH, Liu J, Soker S, Atala A, Yoo JJ, Biomaterials, 29, 1442 (2008)
  •  
  • 12. Mahoney MJ, Saltzman WM, J. Pharm. Sci., 90, 1356 (1996)
  •  
  • 13. Benoit JP, Faisant N, Venier-Juliene MC, Menei P, J. Control. Release, 65, 285 (2000)
  •  
  • 14. Willerth SM, Sakiyama-Elbert SE, Adv. Drug. Deliver. Rev., 59, 325 (2007)
  •  
  • 15. Nihant N, Schugens C, Grandfils C, Jerome R, Teyssile P, Pharm. Res., 11, 1479 (1994)
  •  
  • 16. Schugens C, Laruelle N, Nihant N, Grandfils C, Jerome R, Teyssie P, J. Control. Release, 32, 161 (1994)
  •  
  • 17. Gabor F, Ertl B, Wirth M, Mallinger R, J. Microencapsul., 16(1), 1 (1999)
  •  
  • 18. Sansdrap P, Moes AJ, Int. J. Pharm., 98, 157 (1993)
  •  
  • 19. Couvreur P, Blanco-prieto MJ, Puisieux F, Roques B, Fattal E, Adv. Drug. Deliver. Rev., 28, 85 (1997)
  •  
  • 20. Jeyanthi R, Mehta RC, Thanoo BC, DeLuca PP, J. Microencapsul., 14(2), 163 (1997)
  •  
  • 21. Cohen S, Yoshioka T, Laucarelli M, Hwang LH, Langer R, Pharm. Res., 8, 713 (1991)
  •  
  • 22. Meinel L, Illi OE, Zapf J, Malfanti M, Merkle HP, Gander B, J. Control. Release, 70, 193 (2001)
  •  
  • 23. So JW, Kim SH, Baek MO, Lim YY, Roh HW, Lee NR, Kim MS, Ryu GH, Cho Y, Lee SJ, Min BH, Khang G, Lee HB, Tissue Eng. Regen. Med., 4, 577 (2007)
  •  
  • 24. Jeffery H, Davis SS, O'Hagan DT, Pharm. Res., 10, 362 (1993)
  •  
  • 25. Sah H, Toddywala R, Chien YW, J. Control. Release, 30, 201 (1994)
  •  
  • 26. Marchais H, Boury F, Damge C, Proust JE, Benoit JP, S.T.P. Pharma. Sci., 6, 417 (1996)
  •  
  • 27. Herrmann J, Bodmeier R, Int. J. Pharm., 126, 129 (1995)
  •  
  • 28. Park JS, Yang JC, Yuk SH, Shin HS, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(3), 189 (2007)
  •  
  • 29. Lyons SL, Wright SG, US Patent 6,331,317, (2001)
  •  
  • 30. Kim SK, Kim SH, Lee HR, Cho MH, Kim MS, Khang G, Lee HB, Tissue Eng. Regen. Med., 2, 388 (2005)
  •  
  • 31. Kim SM, Kim SH, Kim CM, Oh AY, Kim GA, Lee IW, Rhee JM, Khang G, Kim MS, Lee HB, Tissue Eng. Regen. Med., 4, 589 (2007)
  •  
  • 32. Kim SM, Kim SH, Kim CM, Oh AY, Lee IW, Kim MS, Rhee JM, Khang G, Lee HB, Tissue Eng. Regen. Med., 4, 583 (2007)
  •  
  • 33. Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)
  •  
  • 34. Goraltchouk A, Scanga V, Morshead CM, Shoichet MS, J. Control. Release, 110, 400 (2006)
  •  
  • 35. Yang YY, Chung TS, Bai XL, Chan WK, Chem, Eng, Sci., 55, 2223 (2002)
  •  
  • 36. Lee HY, Lee HS, Kim JS, Kim SB, Lee JS, Choi HI, Chang SG, International Patent WO 01/78687 A1 (2001)
  •  
  • 37. Carrasquillo KG, Stanley AM, Aponte-carro JC, Jesus PD, Costantino HR, Bosques CJ, Grebenow K, J. Control. Release, 76, 199 (2001)
  •  
  • 38. Lee JI, Yoo HS, Colloids Surf. B Biointerfaces, 61, 81 (2008)
  •  
  • 39. O'Mahony M, Sensory Evaluation of Food: Statistical Methods and Procedures, CRC Press, p. 487 (1986)
  •  
  • 40. William H, Teukolsky SA, Vetterling WT, Flannery BP, Numerical Recipes in C: The Art of Scientific Computing, Cambridge University Press, p. 616 (1992)
  •  
  • 41. Khang G, Lee HB, Biomedical Polymers, p.19 (2001)
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2009; 33(1): 7-12

    Published online Jan 25, 2009

  • Received on Apr 24, 2008
  • Accepted on Aug 6, 2008