Article
  • Ultrasonic Velocity and Absorption Measurements in Gel of Proteins
  • Bae JR
  • 초음파에 의한 단핵질 Gel화의 연구
  • 배종림
Abstract
Ultrasonic velocity and absorption measurement were made at 3 MHz in ovalbumin, conalbumin and ovomucoid which were protein constituent of egg white, over temperature range 10∼95℃ using pulse echo overlap method. The gelation process caused by heat was studied by the change in the velocity and absorption. The gelation temperatures of the ovalbumin and conalbumin proteins were 70℃ and 50℃, respectively. However the gelation of the ovomucoid protein did not occur in gelation under experimental temperature condition. The change in the absorption and velocity due to gelation was observed as a function of time at aging temperatures ranging front 50∼95℃ for conalbumin, 70∼95℃ for ovalbumin. The experimental results showed that the change were prominent at 55℃ in conalbumin, 75℃ in ovalbumin. Hysteresis phenomenon was also observed by varying the aging temperatures as a parameter. This phenomenon is associated with the memorizing effect of the network structure of protein in gel. It was demonstrated that the ultrasonic technique was useful for monitoring the gelation process.

Egg white의 gel화에 따른 음속과 흡수의 변화가 60°와 75℃에서 크게 나타난 것이 egg white의 어느 단백질성분에 의한 것인가를 조사하기 위해 egg white의 주요한 단백질성분인 ovalbumin, conalbumin, ovomucoid protein에 대해 gel화에 따른 음속 및 흡수의 변화를 온도 10∼95℃의 범위에서 초음과 pulse법을 사용하여 측정하였다. Ovalbumin은 70℃, conalbumin은 50℃에서 gel화가 시작되었고 ovomucoid는 측정온도범위에서는 gel화가 진행되지 않았다. Gel화하는 이상의 온도에서 음속과 횹수에 대하여 aging측정을 행하여 gel화에 의한 음속과 흡수의 변화를 관측하였다. 그 결과 conalbumin은 50℃, ovalbumin는 75℃에서 음속과 흡수의 변화가 많이 일어났다. Egg white의 60°와 75℃의 gel화에 의한 음속과 흡수의 큰 변화는 각각 conalbumin과 ovalbumin에 의한 것임을 알았고 Conalbumin과 ovalbumin는 aging 온도를 parameter로 하여 이력현상이 관측되었다.

References
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  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 1994; 18(1): 119-125

    Published online Jan 25, 1994