Article
  • Cross Linked Polyvinyl Alcohol Embedding Resin by Boric Acid and NaOH for Cryomicrotomy

  • Jin Ik Lim†

  • Department of Chemical Engineering, College of Engineering, Dankook University, Jukjeon-dong, Yongin-si, Gyeonggi-do 16890, Korea

  • 동결절편을 위해 붕산과 NaOH에 의해 가교된 폴리비닐알코올 포매용 레진

  • 임진익†

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

References
  • 1. Lee, K. J. D.; Knox, P. J. Resin Embedding, Sectioning, and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues. Methods Mol. Biol. 2014, 1080, 41-52.
  •  
  • 2. Yahiro, J.; Nagato, T. Application of Ion Etching to Immunoscanning Electron Microscopy. Microsc. Res. Tech. 2005, 67, 240-247.
  •  
  • 3. Murray, G. I.; Burke, M. D.; Ewen, S. W. Enzyme Histochemistry on Freeze-dried, Resin-embedded Tissue. J. Histochem. Cytochem. 1989, 37, 643-652.
  •  
  • 4. Britten, K. M.; Howarth, P. H.; Roche, W. R. Immunohistochemistry on Resin Sections: a Comparison of Resin Embedding Techniques for Small Mucosal Biopsies. Biotech. Histochem. 1993, 68, 271-280.
  •  
  • 5. Mondon, J. A.; Howitt, J.; Tosiano, M.; Kwok, K. W. H.; Hinton, D. E. A Simple Osmium Post-fixation Paraffin-embedment Technique to Identify Lipid Accumulation in Fish Liver Using Medaka (Oryziaslatipes) Eggs and Eleutheroembryos as Lipid Rich Models. Mar. Pollut. Bull. 2011, 63, 86-90.
  •  
  • 6. Schneider, G.; Voigt, S.; Alde, A.; Berg, A.; Linde, D.; Otto, K.; Enkelmann, A.; Schnabelrauch, M. Iodine Doping of a Photocuring, One-component Embedding Medium for µCT and Histology. Br. J. Radiol. 2021, 94, 20210129.
  •  
  • 7. Nolta, N. F.; Liberti, A.; Makol, R.; Han, M. Gelatin Embedding and LED Autofluorescence Reduction for Rodent Spinal Cord Histology. J. Neurosci. Methods 2020, 1, 108924.
  •  
  • 8. McDonald, K. L. Rapid Embedding Methods Into Epoxy and LR White Resins for Morphological and Immunological Analysis of Cryofixed Biological Specimens. Microsc. Microanal. 2014, 20, 152-163.
  •  
  • 9. Erben, R. G. Embedding of Bone Samples in Methylmethacrylate: An Improved Method Suitable for Bone Histomorphometry, Histochemistry, and Immunohistochemistry. J. Histochem. Cytochem. 1997, 45, 307-313.
  •  
  • 10. Nagashima, K.; Zheng, J.; Parmiter, D.; Patri, A. K. Biological Tissue and Cell Culture Specimen Preparation for TEM Nanoparticle Characterization. Methods Mol. Biol. 2011, 697, 83-91.
  •  
  • 11. Ticha, P.; Pilawski, I.; Yuan, X.; Pan, J.; Tulu, U. S.; Coyac, B. R.; Hoffmann, W.; Helms, J. A. A Novel Cryo-embedding Method for In-depth Analysis of Craniofacial Mini Pig Bone Specimens. Sci. Rep. 2020, 10, 19510.
  •  
  • 12. Cohen, M.; Varki, N. M.; Jankowski, M. D.; Gagneux, P. Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution. J. Vis. Exp. 2012, 21, e3928.
  •  
  • 13. Bronson, D. D.; Bidmon, H. J.; Iomaschko, K. H.; Stumpf, W. E. Novel Applications of Acrylamide for Cryosectioning of Isolated Cells, Tissues, and Arthropods. J. Histochem. Cytochem. 1991, 39, 1289-1293.
  •  
  • 14. Gavin, T.; Loryn, S. The Use of Optimal Cutting Temperature Compound Can Inhibit Amplification by Polymerase Chain Reaction. Diagn. Mol. Pathol. 1997, 6, 298-303.
  •  
  • 15. Lim, J. I.; Park, H. K. Boric Acid-enhanced Embedding Medium for Cryomicrotomy. Acta Histochem. 2012, 114, 296-299.
  •  
  • 16. Clark, H.; Gorlin, R. J. On the Use of a Modified Fifteen-minute Hematoxylin and Eosin Tissue Stain. Oral Surg. Oral Med. Oral Pathol. 1985, 11, 431-432.
  •  
  • 17. Jakubovský, J.; Guller, L.; Černá, M.; Balážová, K.; Polák, S.; Jakubovská, V.; Babál, P. Fluorescence of Hemaotoxylin and Eosin-stained Histological Sections of the Human Spleen. Acta Histochemica 2002, 10, 353-356.
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2023; 47(4): 447-452

    Published online Jul 25, 2023

  • 10.7317/pk.2023.47.4.447
  • Received on Feb 11, 2023
  • Revised on Mar 31, 2023
  • Accepted on Apr 16, 2023

Correspondence to

  • Jin Ik Lim

  • Department of Chemical Engineering, College of Engineering, Dankook University, Jukjeon-dong, Yongin-si, Gyeonggi-do 16890, Korea

  • E-mail: limjinik@dankook.ac.kr