Copolymerization of diallyl ether(DAE) and maleic anhydride(MAH) were carried out in benzene at 60℃ with 2,2''-Azobisisobutyronitrile(AIBN). The copolymers were characterized by IR and NMR spectroscopy, GPC and TGA. The copolymer composition was determined by elemental analysis. The copolymer had alternating structure. The formation of charge-transfer(CT) complex was identified during the copolymerization of DAE, and MAH, where the equilibrium constant of the CT complexation was estimated as 0.74 liter/mole. For biological activity, the cytotoxicity of the copolymer obtained and natural killer(NK) activity of th(: polymer-treated Lymphocytes on cells were evaluated with K562 leukemia cell line. It was found that the copolymer showed no cytotoxicity below the concentration of 1.0 mg/ml but augmented NK activity of lymphocytes. It was also observed that the cytotoxicity was increased with increasing amount of the copolymer.

Diallyl ether(DAE)와 maleic anhydride:(MAH)를 용매로서 벤젠을 사용하여 60℃에 서 라디칼 공중합을 하였다. 중합체들의 특성은 IR. NMR, GPC 및 TGA등으로 결정하였으며, 중합체내의 단량체조성은 원소분석으로 구하였다. 공중한체는 교호구조를 가지며, 공중합시 두 단량체간에는 UV스펙트럼에 의해 charge-transfer complex가 형성됨을 확인하였로, 이때의 평형상수값은 0.74 liter/mole이었다. 공중합체의 생물학적인 활성은 K562 세포에 대한 공중합체 자체의 세포독성과 공중합체로 처리된 림프구의 자연세포 살해능으로써 조사하였다. 그 결과 공중합체 자체는 1.0mg/ml농도 이하에서 세포독성이 없었으나 림프구의 자연세포 살해능을 활성화시키는 효과를 보였으며, 자연세포 살해능은 공중합체 농도가 증가할수록 증가하였다.

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