The gelatin hydrogel modified with tyramine (Gel-Tyr) was prepared an enzymatic crosslinking reaction to develop a sprayable wound dressing that is easy to use and hygienic on broad and complicate wound area with biocompatibility, wound healing ability, and comfortable wearing. The substitution rate of Gel-Tyr was calculated through ¹H NMR. And the gelation time according to the concentration of gelatin, horseradish peroxidase (HRP) and H₂O₂, rheological properties, swelling ratio, biodegradation behavior, in vitro cytotoxicity, cell viability was evaluated. The hydrogel with rapid gelation rate and optimum performance as a wound dressing was selected as a sample prepared with a compound concentration of Gel-Tyr 3 wt%, HRP 3.50 units/mL, and H₂O₂ 0.2 μL/mL. In the in vivo full-thickness wound model animal experiment and histological evaluation, the Gel-Tyr hydrogel showed an accelerated wound healing rate and dermal regeneration compared to the control group. It is expected to be used as a sprayable wound dressing in medical treatment, conveniently.

생체적합성, 창상치유능 및 피부밀착성을 가지는 동시에 광범위하고 굴곡진 창상부에 간편하고 위생적으로 적용할 수 있는 스프레이형 창상피복제를 개발하고자 효소촉매 가교반응을 이용하여 티라민으로 개질된 젤라틴(Gel-Tyr) 하이드로젤을 제조하였다. Gel-Tyr의 개질률은 ¹H NMR을 통해 산출하였고 젤라틴, horseradish peroxidase (HRP) 및 H₂O₂의 농도에 따른 하이드로젤의 젤화시간, 유변학적 특성, 팽윤도, 생분해도, in vitro 세포독성, 세포생존거동을 평가하였다. 신속한 젤화속도와 창상피복제로서의 최적성능 조건의 하이드로젤은 배합농도 Gel-Tyr 3 wt%, HRP 3.50 units/mL 및 H₂O₂ 0.2 μL/mL로 제조된 시료로 선정하였다. In vivo 전층창상모델 동물실험 및 조직학적 평가에서 Gel-Tyr 하이드로젤은 대조군보다 촉진된 창상회복속도와 진피재생능을 보여주었으며, 의료현장에서 편리하게 사용할 수 있는 스프레이형 창상피복제로의 활용이 기대된다.

Keywords: gelatin, tyramine, sprayable hydrogel, enzymatic crosslinking, wound dressing.