Article
  • Osteogenic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cell in Several Natural Source Biomaterials/PLGA Hybrid Scaffolds
  • Jeon HY, Kim SM, Jeon YS, Kook YJ, Song JE, Khang GS
  • 여러 가지 천연고분자를 함유한 PLGA 하이브리드 지지체에서 토끼 골수줄기세포의 골분화 능력 평가
  • 전하얀, 김수민, 전유신, 국연지, 송정은, 강길선
Abstract
Poly(lactic-co-glycolic acid) (PLGA) has been most widely used owing to its advantages such as good biodegradability and biocompatibility. Scaffolds made of natural source bio-materials like small intestinal submucosa (SIS), demineralized bone particles (DBP), silk fibroin (SF), and collagen-derived duck's feet collagen (DC) have been proved to be biocompatible. We manufactured composite scaffolds of all these natural biomaterials/PLGA. After making hybrid scaffolds, we measured mechanical strength of scaffolds and observed a porosity and surface morphology of the scaffolds by scanning electronic microscopy. Rabbit bone marrow mesenchymal stem cells (rBMSCs) were cultured, expanded and seeded on scaffolds. Inflammatory responses were checked at 1, 4 and 8 weeks using H&E & Von Kossa staining. DC and SIS group were found to be faster in bone regeneration than other groups. Finally, we have observed that scaffolds using biomaterials are effective in cell attachment, stem cell differentiation, and mineralized tissue formation.

합성고분자인 poly(lactic-co-glycolic acid)(PLGA)는 우수한 생분해능을 가진 생체재료로서 널리 이용되고 있으며, 천연유래 고분자인 실크와 소장 점막하 조직, 탈 미네랄화 골분 그리고 오리발 유래의 콜라겐은 생체 적합한특성을 가지고 있다. 본 논문에서는 여러 가지 천연유래 고분자를 함유한 PLGA를 제작한 후, 지지체의 물성 및 표면을 확인하였으며 토끼 골수유래줄기세포를 이용하여 지지체에 파종 후, 세포의 생존율과 세포분화 정도를 측정하였고 in vivo 실험에서 면역조직학적 염색인 H&E와 Von Kossa을 실시하여 골분화 정도를 확인하였다. 결과적으로 오리발 유래 콜라겐과 소장 점막하 조직을 함유한 지지체에서 빠른 골 분화의 모습을 확인할 수 있었다.

Keywords: collagen; silk; PLGA; scaffold; bone marrow mesenchymal stem cells

  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2017; 41(5): 867-873

    Published online Sep 25, 2017

  • 10.7317/pk.2017.41.5.867
  • Received on Apr 4, 2017
  • Revised on Nov 30, -0001
  • Accepted on May 26, 2017

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