The purpose of this study was to design the gel composed of gellan gum (GLG) and chondroitin sulfate (CH) and to evaluate their efficacy as an anti-adhesion barrier after abdominal surgery. To improve the mechanical properties of the gels, GLG/CH gels were treated in phosphate buffered saline (PBS) and calcium chloride (CaCl2) solutions for cross-linking of polymer chains by divalent cations. The physical and mechanical properties of the gels were confirmed by swelling, gel content, gel strength and fourier transform infrared spectroscopy (FTIR) studies. The positive control, negative control and experiment groups were evaluated for prevention of postoperative adhesion formation in rats. The GLG/CH gel (weight ratio of 3:3) showed high effectiveness for the prevention of peritoneal tissue adhesion. In conclusion, the GLG/CH gel can be a good candidate material as a tissue adhesion barrier.

본 연구는 수술 후 유착방지 기능을 갖는 gellan gum(GLG)과 chondroitin sulfate(CH) 조성젤을 만들고 그 효능을 평가하였다. GLG/CH 조성 젤에 phosphate buffered saline(PBS)와 calcium chloride(CaCl2)를 첨가하여 젤을 가교시켰다. 젤의 특성을 확인하기 위해 팽윤성, 젤화도, 젤강도를 측정하고 FTIR로 특성을 확인하였다. Rat에 양성대조군, 음성대조군, 실험군을 넣어 유착방지 효능을 평가하였다. GLG/CH(3:3) 조성 젤은 복막의 조직 유착방지제로 높은 효과를 나타냈고 조직 유착방지막 소재로 활용 가능성을 보였다.

Keywords: gellan gum; chondroitin sulfate; tissue anti-adhesive; gel