1,4-Butanediol diglycidyl ether (BDDE) crosslinked hyaluronic acid (HA) suspended in three different HAs (697, 1058, 1368 kDa) were prepared to investigate the effect of HA molecular weight on swelling property and elastic modulus of the hydrogels. The amount of the residual BDDE after final dialysis was less than 0.5 ppm. The highest swelling ratio was observed for the uncrosslinked HA having the largest molecular weight. The expansion capacity of HA rose with increasing the amount of pure HA and was inversely proportional to the crosslinking degree due to an increased number of coiled HA chain interactions. Elastic modulus (G') of monophasic fillers having different ratios of 1.0 w/v% BDDE crosslinked HAs to pure HA (1368 kDa) were within 152 and 325 Pa. 178 Pa was observed for the biphasic fillers consisting of 15% crosslinked HA (697 kDa) nanoparticles suspended in 85% of uncrosslinked HA (1368 kDa).

분자량이 다른 세종류 히알루론산(HA)에 1,4-butanediol diglycidyl ether(BDDE)가 가교된 HA를 첨가하여 하이드로겔을 제조하고 HA 분자량에 따른 팽윤도와 탄성계수의 효과를 조사하였다. 최종 투석 후 잔류 BDDE 양은 0.5 ppm 이하이었다. 비가교된 분자량이 큰 HA에서 최대 팽윤도가 관찰되었다. 팽윤도는 비가교 HA 양과 가교도에 각각 비례와 반비례하였다. 1.0 w/v% BDDE 가교 HA와 HA(1368 kDa)를 혼합한 monophasic 하이드로젤의 탄성계수는 152~325 Pa이었다. 15% 가교 HA(687 kDa)과 85% HA(1368 kDa)로 구성된 biphasic 하이드로젤의 탄성계수는 178 Pa이었다.

Keywords: hyaluronic acid hydrogel; dermal filler; 1,4-butanediol diglycidyl ether; crosslink; elastic modulus