Article
  • Cell Co-culture Method by Patterned Graft of Thermo-Responsive Polymer
  • Bae JS, Ahn CH, Yoon KH, Kwon OH, Kang IK, Yamato M, Kikuchi A, Okano T
  • 온도응답성 고분자의 패턴상 그래프트를 이용한 공배양법
  • 배진숙, 안창현, 윤관한, 권오형, 강인규, Yamato M, Kikuchi A, Okano T
Abstract
Thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) was covalently patterned by masked electron beam irradiation. Introduction of PIPAAm on tissue culture polystyrene dish was confirmed by ATR-FTIR and ESCA measurements. Hepatocytes were cultured at 37 ℃ on these surfaces. Cells adhered on PIPAAm-grafted domains were detached by reducing culture temperature to 20 ℃. Endothelial cells were then seeded and cultured on the same surfaces. Seeded endothelial cells were selectively attached on hepatocytes detached and PIPAAm-grafted domains and could be co-cultured with hepatocytes on the same culture dishes with clear pattern. This co-culture method enabled long-term co-culture of hepatocytes with endothelial cells.

온도응답성 고분자인 PIPAAm을 포토마스크를 사용하여 전자빔조사에 의해 패턴상으로 세포배양용 폴리스티렌 접시표면에 그래프트하였다. 폴리스티렌 표면에의 PIPAAm의 그래프트는 ATR-FTIR과 ESCA에 의한 표면분석을 통해 확인하였다. 이러한 표면에 간실질세포를 37 ℃에서 배양하였고, 균일하게 간세포가 배양된 배양접시를 PIPAAm의 LCST 이하인 20 ℃로 배양온도를 낮추어 PIPAAm이 그래프트된 도메인에 접착된 간실질세포를 탈착시키고 배양 접시를 다시 37 ℃로 올린 후 두 번째 혈관내피세포를 파종하여 PIPAAm이 그래프트된 도메인에만 선택적으로 접착시킴으로써 같은 평면상에서 간실질세포와 혈관내피세포를 공배양할 수 있게 되었다. 이러한 방법으로 생체외에서 간실질세포와 혈관내피세포를 장기간에 걸쳐 공배양할 수 있었다.

Keywords: patterning; thermo-responsive polymer; co-culture; endothelial cell; hepatocyte

  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2023 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2005; 29(3): 294-299

    Published online May 25, 2005

  • 10.7317/pk.
  • Received on Feb 28, 2005
  • Revised on Nov 30, -0001
  • Accepted on Apr 7, 2005

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