In order to utilize the LDL binders for the treatment of the type IIa familial hypercholesterolemia (FH) patients, polymeric low density lipoprotein (LDL) binders were prepared by grafting negatively charged sulfonate groups to cellulose and glass beads. The resulting LDL hinders were characterized by FTIR, ¹H-NMR, and elemental analysis and their lipid binding capacity was assessed from human serum. The content of introduced sulfonate groups was 6.6×10^-5∼3.9×10^-4 mol/g, depending on substrates and modification methods. The LDL adsorption to sulfonated binders, in general increased as compared with untreated controls. Especially, dextran sulfate-grafted glass beads (Glass-TEOS-DS ) have shown to decrease LDL level upto 48% among various LDL hinders. Thls result indicates that LDL is selectively and effectively eliminated by a Glass-TEOS-DS, which can be applied for LDL apheresis.

Type Ila 가족성 고콜레스테롤 헐중 환자의 치료를 위해 셀룰로오즈 및 glass beads의 기질에 음이온성 술폰산기를 그라프트시킨 저밀도 지방단백질 (LDL) 제거용 고분자를 제조하였다. 제조된 제거제들은 FTIR, ¹H-NMR 및 원소분석기로 분석하였으며 사람의 헐청으로부터 그들의 LDL 흡착능을 평가하였다. 그라프트된 술폰산기의 함량은 기질 및 개질방법에 따라 6.6×10^-5∼3.9×10^-4 mo1/g 이었으며 LDL 흡착정도는 술폰산화 제거제가 미처리 control에 비해서 상당히 높게 나타났다. 특히 glass beads에 dextran sulfate (DS)를 고정화시킨 Glass-TEOS-DS는 LDL값이 48% 까지 크게 감소하여 효과적으로 LDL을 제거할 수 있음을 확인하였다.

Keywords: polymeric LDL binders; surface modification; LDL opheresis